TAGZyme Kit. For the removal of His tags from proteins with an intrinsic or an introduced DAPase stop point. Kit contents: For processing of approximately 10 mg tagged protein: 0.5 units DAPase Enzyme, 30 units Qcyclase Enzyme, 10 units pGAPase Enzyme, 20 mM Cysteamine-HCl (1 ml), Ni-NTA Agarose (10ml), 20 Disposable Columns.
Benefits: Highly specific exoproteolytic activity prevents internal cleavage. Complete solution for His-tag removal. Efficient tag removal: >95% in just 30 minutes at 37°C. High-purity end products . Subtractive Ni-NTA chromatography to remove all contaminants
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