SuperScript® III Reverse Transcriptase (RT) provides higher yields of full-length cDNA for more complete gene product representation compared to other RT enzymes. Robust cDNA synthesis is a critical component of gene expression research. Suboptimal RT reactions can lead to low yields of cDNA that compromise sensitivity or truncated cDNA that can lead to false negative results. Most reverse transcription enzymes fail to consistently produce cDNA with full representation of input RNA due to significant RNase H activity and limited thermostability. SuperScript® III RT has been engineered for higher thermostability and a longer half-life at 50°C. In addition SuperScript® III exhibits reduced RNase H activity. If you need maximum sensitivity for real time RT-PCR and microarray experiments and cDNA that represents input RNA with maximum fidelity, you need SuperScript® III RT.
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