Subcloning Efficiency™ DH5α™ Competent Cells


Life Technologies

DH5α™ is a well-known, versatile strain that can be used in many everyday cloning applications. In addition to supporting blue/white screening, recA1 and endA1 mutations in DH5α™ increase insert stabi...read more

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Description

 

DH5α™ is a well-known, versatile strain that can be used in many everyday cloning applications. In addition to supporting blue/white screening, recA1 and endA1 mutations in DH5α™ increase insert stability and improve the quality of plasmid DNA prepared from minipreps.

DH5α™ Cells offer the following benefits:

  • A wide range of efficiencies from >1 x 10^6 to >1 x 10^9 transformants/µg
  • Greatly increased plasmid yield and quality due to endA1 mutation
  • Blue/white screening of recombinant clones due to lacZΔM15
  • Ensured insert stability due to recA1 mutation

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