Sphero™ Rainbow Calibration Particles (6 peaks), 3.0-3.4 mm

Manufacturer BD Biosciences

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The vial contains a mixture of 3.0 - 3.4 µm Rainbow Particles that are dyed to six different fluorescent intensities. Every Rainbow Particle contains a mixture of fluorophores that are excited at any wavelength from 365 – 650 nm. The Rainbow Particles have emission spectra compatible with many common fluorophores used for immunofluorescent staining with flow cytometric analysis.

Preparation and Storage

The particle mixture (~ 10 x 106 particles/ml) should be stored undiluted at 4°C and protected from direct exposure to light. Do not freeze.


This particle mixture is useful for routine calibration of flow cytometers. Before use, resuspend the particles by vortexing. Dilution of 3 - 5 drops of particles to 1 ml of sheath fluid will provide an adequate number of particles for flow cytometric analysis. Since applications vary, each investigator must determine dilutions appropriate for individual use.