SingleShot™ Cell Lysis RT-qPCR Kits

Manufacturer Bio-Rad
5.0
/
5.0
  |  1 reviews


Product Image
Buy Now

Place your order directly with the manufacturer.




Average Rating: 5.0
1 Scientist has reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


excellent results
Rating: 5.0

  • Application Area: Gene expression and amplifications

"Very reliable, accurate and worth its costs and values"

Review date: 03 Mar 2017 | SingleShot™ Cell Lysis RT-qPCR Kits
The SingleShot family of kits provides a complete and fast solution for generation of lysates from cell cultures, optimized for downstream one- or two-step quantitative PCR (qPCR) reactions, without an RNA purification step.

Simple, short, minimal hands-on protocol is suitable for automation and provides a high-throughput solution. Grow cells to desired density, transfer up to 105 cells to wells of a PCR plate, add lysis solution, and incubate at room temperature for 10 minutes. Heat for 5 minutes at 37°C and then for 5 minutes at 75°C. The lysate is ready for RT-qPCR or can be stored for future use.

Kits are available in SYBR® Green or probe chemistries. A cell lysis kit is also offered as a stand-alone option.


Features:

  • Cell lysis for RT-qPCR
  • RT-qPCR reagents sold separately
  • No RNA purification needed

Benefits:

  • Cell lysate from 10 to 100,000 cells ready in 20 minutes
  • Complete removal of genomic DNA while preserving RNA integrity
  • Optimal accuracy and linear dynamic range of qPCR data across genes
  • Results comparable to using purified RNA, including dynamic range
  • Validated with PrimePCR™ Assays and Panels
  • SingleShot RNA Control ensures optimal cell and lysate inputs