SingleShot™ Cell Lysis RT-qPCR Kits

SingleShot™ Cell Lysis RT-qPCR Kits
by Bio-Rad


The SingleShot family of kits provides a complete and fast solution for generation of lysates from cell cultures, optimized for downstream one- or two-step quantitative PCR (qPCR) reactions, without an RNA purification step. Simple, short, minimal hands-on protocol is suitable for automation and provides a high-throughput solution. Grow cells to desired density, transfer up to 105 cells to we...read more

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Description

 

The SingleShot family of kits provides a complete and fast solution for generation of lysates from cell cultures, optimized for downstream one- or two-step quantitative PCR (qPCR) reactions, without an RNA purification step.

Simple, short, minimal hands-on protocol is suitable for automation and provides a high-throughput solution. Grow cells to desired density, transfer up to 105 cells to wells of a PCR plate, add lysis solution, and incubate at room temperature for 10 minutes. Heat for 5 minutes at 37°C and then for 5 minutes at 75°C. The lysate is ready for RT-qPCR or can be stored for future use.

Kits are available in SYBR® Green or probe chemistries. A cell lysis kit is also offered as a stand-alone option.


Features:

  • Cell lysis for RT-qPCR
  • RT-qPCR reagents sold separately
  • No RNA purification needed

Benefits:

  • Cell lysate from 10 to 100,000 cells ready in 20 minutes
  • Complete removal of genomic DNA while preserving RNA integrity
  • Optimal accuracy and linear dynamic range of qPCR data across genes
  • Results comparable to using purified RNA, including dynamic range
  • Validated with PrimePCR™ Assays and Panels
  • SingleShot RNA Control ensures optimal cell and lysate inputs

Product Overview

SingleShot™ Cell Lysis RT-qPCR Kits by Bio-Rad
SingleShot™ Cell Lysis RT-qPCR Kits

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