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Member since: 2016
Organization: Montclair State University Dept of Biology
Wonderful teaching tool.
Application Area: The cellular effects of the dust from the 9-11 World Trade Center (WTC) disaster
"We have used several assays over the past years. They have become a wonderful teaching tool and clear way to pin point changes in cell activity caused by the sample of dust from 9-11. Thank you PROMEGA you have been at my right hand; with your help I have been able to allow my creative Genotox students prepare for this 21st Century!!!"
This assay allows identification of conditions or test compounds, such as small molecule inhibitors or inducers, that alter ROS levels. The scalable multiwell format couples a stable luminescent signal to the level of H2O2 in a sample.
An H2O2 Substrate is employed that reacts directly with H2O2 to generate a luciferin precursor. Upon addition of ROS-Glo™ Detection Reagent containing Ultra-Glo™ Recombinant Luciferase and D-Cysteine, the precursor is converted to luciferin by the D-Cysteine, and the produced luciferin reacts with Ultra-Glo™ Recombinant Luciferase to generate a luminescent signal that is proportional to H2O2 concentration.
ROS-Glo™ H2O2 Assay Benefits: