Analtech Reversed Phase Separation (RPS) UNIPLATES offers several advantages over the bonded RP18 type TLC plates. RBS UNIPLATES are a hydrocarbon impregnated silica gel. The hydrocarbon is adsorbed onto the silica gel surface and is not chemically anchored to the particle.
The main advantages of the hydrocarbon impregnated Reversed Phase plate are compatibility with aqueous solutions and parallel behavior to most C18 HPLC packings. The RPS UNIPLATE will accept 100% water either as the mobile phase or spotting solvent. This is a great advantage when working with polar sample components such as proteins and peptides.
RBS UNIPLATES are also less expensive than bonded phase TLC plates, have aqeuous compatibility for polar samples, are an inorganic binder, perform rapid, inexpensive methods in development for HPLC, and have expanded flexibility to a wide range of applications.
The RPS impregnated UNIPLATE approximates the adsorption characteristics of the bonded C18 modified silica gels. Therefore, RPS Reversed Phase UNIPLATES are an excellent medium for rapid, inexpensive screening of chromatographic conditions in HPLC method development. Mixtures of water or buffers plus a polar organic modifier (eg. Methanol, acetonitrile, THF, etc.) are easily scouted with RPS UNIPLATES. However, since the hydrocarbon is adsorbed but not chemically anchored to the silica gel, the RPS UNIPLATES cannot tolerate nonpolar organic solvents. Ethyl Acetate or less polar organic solvents will strip the hydrocarbon from the silica get support. (For organic solvent compatible plates see the UNIBOND series of HPTLC plates.)