Recombinant Human SCF

Recombinant Human SCF
by R&D Systems, Inc.


R&D Systems Recombinant SCF Protein Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28 - 40 kDa type I transmembrane glycoprotein. It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenito...read more

Request Pricing




Receive your quote directly from R&D Systems, Inc. for Recombinant Human SCF

Description

 

R&D Systems Recombinant SCF Protein

Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28 - 40 kDa type I transmembrane glycoprotein. It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors.

Specifications and Use for R&D SCF 

Source of R&D SCF

• A DNA sequence encoding the extracellular domain human SCF (Glu 26 - Ala 189; Accession # 134289) (Martin, F.H. et al., 1990, Cell 63:203 - 211) was expressed in E. coli.

Molecular Mass of Recombinant SCF

• The 165 amino acid residue recombinant protein has a predicted molecular mass of approximately 18.6 kDa.

Purity of R&D SCF

• > 97%, as determined by SDS-PAGE and visualized by silver stain.

Endotoxin Level
• < 1.0 EU per 1 μg of the cytokine as determined by the LAL method.

Activity of R&D SCF

• The biological activity of recombinant human stem cell factor was measured in a cell proliferation assay using a human factor-dependent cell line, TF-1 (Kitamura, T. et al., 1989, J. Cell Physiol. 140:323 - 334).
• The ED50 for this effect is typically 2.5 - 10 ng/mL. The cell number is assessed in a fluorometric assay using the redox sensitive dye, Resazurin (R&D Systems, Catalog # AR002).

Formulation
• Lyophilized from a 0.2 μm filtered solution in PBS containing 50 μg of bovine serum albumin per 1 μg of cytokine.

Reconstitution of R&D SCF  
• It is recommended that sterile phosphate-buffered saline containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution containing no less than 10 μg/mL of cytokine.

Storage of R&D SCF
• Lyophilized samples are stable for up to twelve months from date of receipt at -20° C to -70° C.
• Upon reconstitution, this cytokine can be stored under sterile conditions at 2° - 8° C for one month or at -20° C to -70° C in a manual defrost freezer for three months without detectable loss of activity.
• Avoid repeated freeze-thaw cycles.

Product Overview

Recombinant Human SCF by R&D Systems, Inc.
Recombinant Human SCF

SelectScience Trusted Banner
Why make an inquiry with SelectScience?

Advertisement