RapiGest™ SF Protein Solubilization Reagent by Waters

Manufacturer Waters
4.5
/
5.0
  |  38 reviews


RapiGest™ SF Protein Solubilization Reagent by Waters product image
RapiGest™ SF Protein Solubilization Reagent
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Average Rating: 4.5
38 Scientists have reviewed this product

5 out of 5
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money


Very handy stuff!! Yields good results
Rating: 4.7

  • Application Area: Proteomics

"It really helps to solve a lot of sample prep issues and alongside fosters efficient digestion."

Review date: 02 Oct 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: MNTA Pharmaceuticals



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
A great tool for producing high quality data for complex analyses
Rating: 4.0

  • Application Area:Antibody digestion for peptide level analysis via LC-MS

"Provides an easy to use option to help produce more complete, reproducible digests."

Review date: 29 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Syngenta Crop Protection



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    3 out of 5
I am going to try it one more time
Rating: 3.7

  • Application Area:Digestion of surface proteins

"Initial results look promising but I haven't been able to reproduce it."

Review date: 29 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Regeneron Pharmaceuticals Inc.



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Fantastic tool for N-glycan analysis, sped up our digestion times by 500%!
Rating: 5.0

  • Application Area:Rapidly digest mABs for N-glycan analysis

"The RapiGest SF Surfactant is an excellent denaturation reagent and cut the total analysis time for our QC Release assay for N-glycan analysis down from 16 hours to only a few hours!"

Review date: 29 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Zoetis Inc.



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
RapiGest gives a better digest for select mAbs, but with some non-specific fragments.
Rating: 4.0

  • Application Area:Non-reduced solution digestion of mAbs for identity confirmation by peptide mapping

"For select mAbs non-reduced, RapiGest produces more complete digestion than digestion in buffer or in combination with an organic solvent. However, expect to observe some undesired non-specific fragmentation."

Review date: 29 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Food and Drug Administration



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Useful
Rating: 5.0

  • Application Area:study of secretome of MSCs

"Rapigest definitely is helping to my sample preparation while trypsin digestion. Easy to use and easy to remove after precipitation. I haven't used filtration of my sample since I can clearly remove the precipitate."

Review date: 29 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Amgen



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Easy to use and reproducible results
Rating: 5.0

  • Application Area:Analyze biologics of different modalities (mAb, Fusion protein, BiTE, etc) by peptide mapping

"The product has been effective in providing the desired results with reduced and non-reduced peptide mapping for different biologic products with reproducible results."

Review date: 27 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: BMS



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Easy to use!
Rating: 5.0

  • Application Area:protein sample

"Easy to use, saves time, reliable I use it for N-linked Glycan Profiling Analysis of Protein use , using HILIC UPLC."

Review date: 25 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: KBI Biopharma



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Very effective and easy to use.
Rating: 5.0

  • Application Area:Glycan profiling, peptide mapping

"The reagent provides very effective denaturation of proteins for peptide map analysis. The protocols are easy to follow. We also use this as part of the RapiFluor MS kit for glycan profiling as a protein denaturant. It is very effective in this regard."

Review date: 24 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Bristol-Myers Squibb



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Excellent reagent for Glycan profiling
Rating: 5.0

  • Application Area:Glycan profiling of mAbs

"Very easy to use, yields high quality and consistent results."

Review date: 22 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent


  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Ardelyx



  • Ease of use
    5 out of 5
    After sales service
    2 out of 5
    Value for money
    1 out of 5
Good results, no complaints.
Rating: 2.7

  • Application Area:proteomics

"Good product. More expensive than other detergents, but it was very easy to use and did not interfere with the LC-MS analysis."

Review date: 21 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Catalent Pharma Solutions



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Great results seen so far!
Rating: 4.0

  • Application Area:biophysical protein characterization

"Great results seen so far! Column has bee easy to use and effective for our purposes."

Review date: 21 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Omeros Corporation



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Easy preparation and removal from the sample mixture
Rating: 5.0

  • Application Area:preparation of slightly basic buffer for the enzymatical protein digestion

"RapiGest SF is very easy to use for the application, and easily removed (or clean-up) from the system, this makes the procedure simple and more efficient for HPLC analysis afterwards . As far as for my application, the assay reproducibility is very satisfactory."

Review date: 21 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: University of Washington



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great service, great product!
Rating: 5.0

  • Application Area:Proteomics

"I am the purchaser of this product for our lab and have received positive feedback from lab members on its ease of use and reproducibility."

Review date: 21 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Georgetown University



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    3 out of 5
For certain application or hard to solubilize samples it is the far best reagent.
Rating: 4.0

  • Application Area:Analyze glycoproteins in purified or complex samples, analyze labeled glycans from complex and purif

"RapiGestSF is so far the best solubilizing agent for most of the applications in glyco- and proteomics. The only drawback is that it's concentration needs to be high, which is decreasing the value for money. For regular day to day proteomic analyses there are other comparable reagents on the market that are used in 10x or even less final concentration."

Review date: 21 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: TSRI



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Great! Will use it for many more years to come!
Rating: 4.0

  • Application Area:Protein digestion for mass spectrometry

"I have used this product for years and like it as it can easily resolubilize my protein of interest after precipitation and is a very good alternative to urea."

Review date: 21 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Stony Brook University



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Worked well, good results
Rating: 4.3

  • Application Area:Immunoprecipitation

"My lab required a denaturant compatible to downstream MALDI-TOF applications where typical detergents such as SDS weren't feasible. RapiGest SF worked well and provided us with good results."

Review date: 21 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: Fujirebio Diagnostics Inc



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
After an established history with Rapigest I wouldn't hesitate to recommend it.
Rating: 4.7

  • Application Area:Characterization of cancer antibodies and antigens.

"The RapiGest SF reagent is fast, effective, and comprehensive. I've found it to work on a variety of antibodies and antigens; requiring no changes to the method or use of the reagent itself. The results are repetitive and precise. We are going to expand it's use outside of Glycan analysis and apply it to a number of assays that have proven problematic with a number of our proprietary proteins."

Review date: 21 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Pfizer



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Great detergent for tissues and membrane proteins. Easy to get rid of.
Rating: 4.0

  • Application Area:In solution digestion prior to ms

"I love to use Rapigest for any in solution digestion, from FFPE to cell lysate."

Review date: 21 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: GSK



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
Great. I would recommend the product
Rating: 4.0

  • Application Area:biologics characterization

"We use RapiGest in the denaturation step to prepare proteomics and peptide mapping samples. it is easy to use. We acquired high quality data. We didn't have a lot of experience on after sales care, since we didn't encounter any issue with the product."

Review date: 21 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Duke University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Great product for high sensitivity analysis by Mass Spectrometry
Rating: 4.7

  • Application Area:Analyze post-translational modifications on purified proteins from plant tissues

"This is a great substitute of other ionic detergents, such as SDS, that are not amenable for mass spectrometry. For my applications, the solubilization effectiveness was almost as good as SDS and its removal by acid treatment was excellent. This product is very helpful, especially when your sample is limiting and you need high sensitivity. I find it somewhat expensive, especially if you need to scale up, but is is worth the cost."

Review date: 21 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Yale University



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Important step to enrich for protein without contaminant.
Rating: 4.3

  • Application Area:To send for shotgun proteomics

"Easy to use as the instructions are thorough making the product paramount for protein isolation."

Review date: 21 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: biomerieux



  • Ease of use
    4 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
Solid product. will help you get your work done.
Rating: 3.7

  • Application Area:protein analysis

"The RapiGest reagent worked great for the protein work that I am currently doing. it got the protein in solution so I could digest it and run it on our triple TOF mass spec. Cost was relatively low for the product and would definitely purchase again."

Review date: 21 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Mallinckrodt Pharmaceuticals



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    3 out of 5
Necessary for methodology
Rating: 4.3

  • Application Area:protein digestion

"I obtained very consistent results while using this product"

Review date: 20 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Systimmune



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Excellent, this product is awesome!
Rating: 5.0

  • Application Area:N release glycan

"This product is very helpful, we can denature the protein more efficiently and use less enzyme to release the N-glycan."

Review date: 12 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Biogen



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Great reagent!
Rating: 4.7

  • Application Area:Protein characterization intact mass analysis

"It is helpful for protein deglycosylation and intact mass analysis. Since it is mass spec compatible, the solution can be directly injected to mass spec for intact mass analysis. This is especially useful when protein denature is required to remove some N-link glycans."

Review date: 12 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Genentech



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Essential product for biopharmaceutical R&D
Rating: 5.0

  • Application Area:Peptide mapping of monoclonal antibodies

"The product provides excellent reproducibliity for generating proteolytic digests from monoclonal antibody."

Review date: 11 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: UTHSCSA



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
RapiGest™ SF Protein Solubilization Reagent is now a permanent addition to our lab.
Rating: 5.0

  • Application Area:Proteomics

"Ease of use and improved results combined with an affordable price make this item a 5-star winner!"

Review date: 11 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: Repligen



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Great and reproducible
Rating: 4.7

  • Application Area:Protein quality

"Great value and ease of use. The prep time is only 30 minutes."

Review date: 11 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Yale University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
I love this product! I don't perform my experiments without it!
Rating: 5.0

  • Application Area:Extract and digest proteins from neurons

"This product has been extremely helpful in the extraction of proteins from my neuron samples. Using RapiGest, I am able to digest the protein immediately after extraction without buffer exchange. RapiGest precipitates nicely out of solution prior to desalting the peptides and does not interfere with downstream mass spectrometry analysis. I would highly recommend this product to anyone performing tissue lysis prior to mass spectrometry analysis."

Review date: 08 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Quality



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Tried it and found the results were good.
Rating: 4.7

  • Application Area:Glycan analysis

"Tried it and found the results were good, but company wanted us to stick to the current sample preparation method. We therefore don't have too much experience with this product."

Review date: 08 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Alexion Pharmaceuticals



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Awesome product!
Rating: 4.7

  • Application Area:Protein therapeutic characterization

"Great product. Easy to use and easy to edit protocol to fit other needs. Great end results."

Review date: 07 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: NIST



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Very positive about this product
Rating: 4.7

  • Application Area:Protein sample processing for mass spectrometry

"Straightforward to use and gives good reproducible mass spectrometry measurement. It would be nice to have it for lower price since sometimes we have to upscale proteins samples and it becomes expensive."

Review date: 06 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: The University of Iowa



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Definitely helped with our protein isolation and our results
Rating: 5.0

  • Application Area:For proteomics.

"Yes, we believe Rapigest helped in our protein isolation and proteomics analysis. The only thing what we noticed is amount of protein isolated was low compared other protein isolation methods."

Review date: 06 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Biogen



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    3 out of 5
very good product
Rating: 4.0

  • Application Area:Protein Surfactant

"Very good surfactant especially on the protein level. However, if used for peptide digestion causes significant non-specific cleavages."

Review date: 06 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Henlix, Inc.



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Great product. Achieved high quality result!
Rating: 4.3

  • Application Area:glycan analysis in monoclonal antibodies

"It increased the efficiency of enzyme digestion or reduction of the antibodies. The instruction is very simple and clear to understand."

Review date: 06 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Moderna



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Reproducible results in variety of matrixes.
Rating: 5.0

  • Application Area:Analyse tissue samples and drug product samples

"The product allows me to obtain reproducible results at high quality. The most important benefit of this product is that it allows me to analyze samples with various degrees of contamination from RNA. It is a great fit for projects where the classical proteomics homogenization/digestion approaches fail. Provides a greater recovery from less soluble peptides."

Review date: 05 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Advanced Reviewer

  • Member since: 2013

  • Organization: Reliance Lifesciences Pvt Ltd



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 4.7

  • Application Area:Digestion of protein for characterization.

"It has characterized items well with right quantities of digestion enzyme. Easy to use. "

Review date: 11 Dec 2014 | RapiGest™ SF Protein Solubilization Reagent
The patented RapiGest™ SF Surfactant is a reagent used to enhance enzymatic digestion of proteins, both in-gel and in-solution. RapiGest SF helps solubilize proteins, making them more susceptible to enzymatic cleavage without inhibiting enzyme activity.

Unlike other commonly used denaturants, such as SDS or urea, RapiGest SF does not modify peptides or suppress protease activity. It is compatible with enzymes such as Trypsin, Lys-C, Asp-N, Glu-C, PNGaseF and other enzymes.
  • Compatible for LC and MS analysis
  • Improves efficiency and speed of digestion
  • Degraded at low pH and removed from the sample
  • Simplifies preparation protocols and improves throughput of analysis

US Patent No: 7,229,539 and US 8,580,533

Product Overview

RapiGest™ SF Protein Solubilization Reagent by Waters product image

RapiGest™ SF Protein Solubilization Reagent

Manufacturer Waters

4.5 / 5.0 | 38 reviews