RapiGest™ SF Protein Solubilization Reagent by Waters

Manufacturer Waters
4.8
/
5.0
  |  13 reviews


RapiGest™ SF Protein Solubilization Reagent by Waters product image
RapiGest™ SF Protein Solubilization Reagent
Request Pricing

Receive your quote directly from the manufacturer.




Average Rating: 4.8
13 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


Excellent, this product is awesome!
Rating: 5.0

  • Application Area: N release glycan

"This product is very helpful, we can denature the protein more efficiently and use less enzyme to release the N-glycan."

Review date: 12 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Biogen



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Great reagent!
Rating: 4.7

  • Application Area:Protein characterization intact mass analysis

"It is helpful for protein deglycosylation and intact mass analysis. Since it is mass spec compatible, the solution can be directly injected to mass spec for intact mass analysis. This is especially useful when protein denature is required to remove some N-link glycans."

Review date: 12 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Genentech



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Essential product for biopharmaceutical R&D
Rating: 5.0

  • Application Area:Peptide mapping of monoclonal antibodies

"The product provides excellent reproducibliity for generating proteolytic digests from monoclonal antibody."

Review date: 11 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: UTHSCSA



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
RapiGest™ SF Protein Solubilization Reagent is now a permanent addition to our lab.
Rating: 5.0

  • Application Area:Proteomics

"Ease of use and improved results combined with an affordable price make this item a 5-star winner!"

Review date: 11 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: Repligen



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Great and reproducible
Rating: 4.7

  • Application Area:Protein quality

"Great value and ease of use. The prep time is only 30 minutes."

Review date: 11 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Yale University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
I love this product! I don't perform my experiments without it!
Rating: 5.0

  • Application Area:Extract and digest proteins from neurons

"This product has been extremely helpful in the extraction of proteins from my neuron samples. Using RapiGest, I am able to digest the protein immediately after extraction without buffer exchange. RapiGest precipitates nicely out of solution prior to desalting the peptides and does not interfere with downstream mass spectrometry analysis. I would highly recommend this product to anyone performing tissue lysis prior to mass spectrometry analysis."

Review date: 08 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Alexion Pharmaceuticals



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Awesome product!
Rating: 4.7

  • Application Area:Protein therapeutic characterization

"Great product. Easy to use and easy to edit protocol to fit other needs. Great end results."

Review date: 07 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: NIST



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Very positive about this product
Rating: 4.7

  • Application Area:Protein sample processing for mass spectrometry

"Straightforward to use and gives good reproducible mass spectrometry measurement. It would be nice to have it for lower price since sometimes we have to upscale proteins samples and it becomes expensive."

Review date: 06 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: The University of Iowa



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Definitely helped with our protein isolation and our results
Rating: 5.0

  • Application Area:For proteomics.

"Yes, we believe Rapigest helped in our protein isolation and proteomics analysis. The only thing what we noticed is amount of protein isolated was low compared other protein isolation methods."

Review date: 06 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Biogen



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    3 out of 5
very good product
Rating: 4.0

  • Application Area:Protein Surfactant

"Very good surfactant especially on the protein level. However, if used for peptide digestion causes significant non-specific cleavages."

Review date: 06 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent


  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Henlix, Inc.



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Great product. Achieved high quality result!
Rating: 4.3

  • Application Area:glycan analysis in monoclonal antibodies

"It increased the efficiency of enzyme digestion or reduction of the antibodies. The instruction is very simple and clear to understand."

Review date: 06 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Moderna



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Reproducible results in variety of matrixes.
Rating: 5.0

  • Application Area:Analyse tissue samples and drug product samples

"The product allows me to obtain reproducible results at high quality. The most important benefit of this product is that it allows me to analyze samples with various degrees of contamination from RNA. It is a great fit for projects where the classical proteomics homogenization/digestion approaches fail. Provides a greater recovery from less soluble peptides."

Review date: 05 Sep 2017 | RapiGest™ SF Protein Solubilization Reagent
  • Status:

    Advanced Reviewer

  • Member since: 2013

  • Organization: Reliance Lifesciences Pvt Ltd



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 4.7

  • Application Area:Digestion of protein for characterization.

"It has characterized items well with right quantities of digestion enzyme. Easy to use. "

Review date: 11 Dec 2014 | RapiGest™ SF Protein Solubilization Reagent
The patented RapiGest™ SF Surfactant is a reagent used to enhance enzymatic digestion of proteins, both in-gel and in-solution. RapiGest SF helps solubilize proteins, making them more susceptible to enzymatic cleavage without inhibiting enzyme activity.

Unlike other commonly used denaturants, such as SDS or urea, RapiGest SF does not modify peptides or suppress protease activity. It is compatible with enzymes such as Trypsin, Lys-C, Asp-N, Glu-C, PNGaseF and other enzymes.
  • Compatible for LC and MS analysis
  • Improves efficiency and speed of digestion
  • Degraded at low pH and removed from the sample
  • Simplifies preparation protocols and improves throughput of analysis

US Patent No: 7,229,539 and US 8,580,533

Product Overview

RapiGest™ SF Protein Solubilization Reagent by Waters product image

RapiGest™ SF Protein Solubilization Reagent

Manufacturer Waters

4.8 / 5.0 | 13 reviews