RapiGest™ SF Protein Solubilization Reagent

RapiGest™ SF Protein Solubilization Reagent

Waters

Waters RapiGest™ SF Surfactant - Enhance enzymatic digestion of proteins. Waters RapiGest SF is a new reagent which radically advances in-solution enzymatic protein digestion in terms o...read more

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Description

 

Waters RapiGest™ SF Surfactant - Enhance enzymatic digestion of proteins.

Waters RapiGest SF is a new reagent which radically advances in-solution enzymatic protein digestion in terms of speed and peptide recovery. RapiGest SF's patented anionic surfactant accelerates the in-solution production of peptides generated by proteases such as trypsin, Asp-N, Glu-C, and Lys-C.

Studies conducted in academic and industrial environments support claims that Waters unique protein solubilization factor (SF), Rapigest SF, dramatically reduces protein digestion times from hours to minutes. 

Waters RapiGest SF is a mild denaturant that helps solubilize and unfold the substrate protein, making it more amenable to cleavage without denaturing or inhibiting common proteolytic enzymes such as trypsin.

Once the samples are generated, a wide variety of Waters instruments can be used in the analysis of the peptides, including Waters HPLC coupled to Micromass® MS Technologies.

Features of the Waters RapiGest SF Protein Solubilization Reagent include:

• Improves efficiency of digestion 
• No adducts or protein modifications observed 
• Degraded at low pH and removed from the sample 
• Compatible with HPLC and MS analysis

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