Can be used to measure very dilute protein concentrations in very small sample volumes. Accurately measures protein concentrations from 0.5 to 30 μg/mL in tube assays and 1 to 20 μg/mL in 96- or 384-well plate assays.
Features and Benefits
• Accurate across a broad range of protein concentrations
• High sensitivity; linear response form 0.5 to 30 μg/ml of protein
• Stable color complex
• Reduced susceptibility to detergents
Based on the alkaline reduction of Cu2+ to Cu1+ by proteins, and the formation of a bicinchoninic acid:Cu1+ complex having an absorbance maximum at 562 nm.