AVOID FREEZE/THAW CYCLES. The ProteoExtract Subcellular Proteome Extraction Kit is a tool for the differential extraction of proteins from adherent or suspension tissue culture cells according to their subcellular localization.
The sequential extraction steps yield fractions containing cytosolic proteins, plasma membrane and organelle proteins, soluble nuclear proteins, and finally cytoskeletal and nuclear matrix proteins.
To preserve the protein profile and increase spot resolution in 2-D gel electrophoresis, the kit also contains Protease Inhibitor Cocktail and the proprietary non-specific Benzonase® Nuclease (Cat. No. 71206). Note: 1 kit is sufficient for up to 20 sample extractions.