Platinum® GenotypeTsp DNA Polymerase is a unique genetically engineered thermostable polymerase (Tsp) which is precomplexed with anti-Tsp antibodies for use in Hot Start PCR. It is designed specifically and functionally tested for amplification of dinucleotide repeat loci in genotyping reactions.
It is genetically engineered such that:
It does not have any 3' exonuclease activity.
It lacks 5' exonuclease activity.
It's extendase activity (tendency to tail DNA with "nontemplate additions") is significantly diminished. Platinum® Genotype Tsp DNA polymerase is engineered so that it can be readily substituted into standard PCR conditions that were designed for Taq DNA polymerase. PCR reactions employing Platinum® Tsp can be set up at room temperature.
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