The HybriZAP-2.1 two-hybrid vector system, a eukaryotic system to detect proteinprotein interactions in vivo, provides a method for the rapid identification of genes encoding proteins that interact with a bait protein. The system is based on the ability to separate eukaryotic transcriptional activators into two separate domains, the DNA binding domain (BD) and the transcriptional activation domain (AD).
Detects protein-protein interactions in vivo using the yeast GAL4 two-hybrid system
Uses a lambda vector for generating high-efficiency cDNA and genomic libraries with high percentage of inserts and increased representation
Easily converts to plasmid format for library screening by in vivo mass excision3
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