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Member since 2013
National Institute Of Nutrition (nin)
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"The quality of the KO mouse from SAGE labs is very good. Since it is well tested and quality controlled, one can rely 100% on these. Sigma is well known for its best after sales services that's always give an edge over other companies. I feel it’s the best in KO animals provider sector."
p53 Knockout Rat from Sigma Advanced Genetic Engineering (SAGE Labs)
Nomenclature: SD- Tp53 tm1sage
This model contains a bi-allelic 14 base pair deletion within exon 3 of the most commonly mutated gene in human cancers, Tp53 (Tumor protein p53). This deletion causes a frameshift in the coding region, resulting in a premature termination codon at the end of exon 3.
Additional Information on the p53 Knockout
p53 is a tumor suppressor protein encoded by the Tp53 gene. Its role in cell cycle regulation and stabilization for preventing genome mutation is observable among a wide variety of multicellular organisms, including humans, rodents, frogs and fish. Homozygous rats deficient in p53 protein are prone to spontaneous tumors, making them valuable for in vivo screening of carcinogenicity, as well as studying chemopreventive and therapeutic treatment.
Characteristics of the p53 Knockout
Total loss of protein via Western blot
Early carcinogenicity screening
p53 function studies