CE-based system for the analysis of nucleic acids and their subsequent interactions with proteins. The system provides clear advantages over traditional electrophoresis for DNA quantitation (viral load, gene expression), synthetic oligonucleotide purity analysis, mutation analysis, size polymorphisms analysis (VNTR, STR, microsatellites, RFLP, RAPD) and the study of DNA/protein interactions. System includes a P/ACE MDQ configured with a selectable-wavelength UV/Vis (200, 214, 254 and 280 nm included) detector, UV source optics, dual-wavelength laser-induced fluorescence detector, 488 nm argon ion laser module, a temperature-controlled sample storage module, and 32 Karat™ Software configured on an IBM personal computer. Installation Qualification, Operation Qualification 1 (OQ1) and documentation to aid in software validation is also included.
Nucleic Acid Analysis
The high resolving power of capillary gel electrophoresis (CGE) and direct UV detection allow the separation and quantitation of oligonucleotides with single base resolution. Illustrated is the separation of a 45-mer oligonucleotide, with clear resolution of the n-1 product
Analysis in solution allows one to easily define an environment to study the interactions of proteins with nucleic acids. Whether research on transcription factors or studies measuring the kinetics of complexation, The P/ACE MDQ Molecular Characterization system provides a platform to assist you in performing these experiments
Quantitation of Gene Expression
Capillary gel electrophoresis with LIF detection accurately quantitates the target and competitor PCR product. Because the separations of these sequences are based on size, confirmation of fragment identity is achieved
Free CD-ROM Tutorial
This CD-ROM tutorial provides seminars on the development of solution-based assays for nucleic acid, protein and carbohydrate analyses. Animation describing these analytical processes is shown, along with practical demonstrations on the implementation of these assays. Our focus is to provide you with tools that improve productivity by cutting through the complexity with simple, bold analytical solutions
Agarose gel electrophoresis (AGE) has been the primary method used to assess the homogeneity of plasmid DNA, however, this approach has some major disadvantages. The AGE method is manual, only semi-quantitative and the assignment of bands to plasmid structures is difficult, as the electrophoretic mobility of plasmids of different shapes changes with the electrophoresis operating conditions A more powerful routine technology for the quantification of plasmid forms is capillary gel electrophoresis (CGE). This automated approach offers high resolution, high sensitivity and high reproducibility to this analysis
Quantitation by Direct Hybridization
CE-LIF provides the sensitivity required for direct hybridization analysis in solution. DNA-probe complexes can be detected at very low levels, removing the need for PCR* amplification. Separation and quantitation of complexes from unbound probes is typically achieved in less than 20 minutes
The automation of gel loading, sample introduction, detection and analysis saves time over conventional genotyping processes. Resolution is sufficient to allow 2-5 bp differences resolved from double-stranded fragments under native electrophoresis conditions, with run-to-run migration time reproducibility typically less than 1.0% R.S.D
Since the first publication of the DNA double helical structure, electrophoresis has been a standard among the analytical tools used for nucleic acid analysis. Capillary electrophoresis (CE) technology extends the power of electrophoresis by using a format that incorporates direct detection, automation and the ability to study interactions in solution. With Beckman Coulter CE technology, you are able to analyze a molecule as it behaves in free solution. This is possible with the high sensitivity of laser-induced fluorescence (LIF) and direct on-line quantitation. With the addition of polymers, the capillary tube can be further transformed into a sieving environment, allowing nucleic acids to be separated by size. A single instrument platform can be used to quantitatively assess oligonucleotide purity, accurately measure gene expression, quantify viral load, study DNA-Protein interactions, and separate nucleic acid fragments for applications such as genotyping and mutation analysis.