TOP10 E. coli are provided at a transformation efficiency of 1 x 10e9 cfu/µg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation. They allow stable replication of high-co...read more
TOP10 E. coli are provided at a transformation efficiency of 1 x 10e9 cfu/µg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation. They allow stable replication of high-copy number plasmids.
The genotype of TOP10 cells is similar to the DH10B™strain, and offers the following features:
hsdR for efficient transformation of unmethylated DNA from PCR amplifications
mcrA for efficient transformation of methylated DNA from genomic preparations
lacZΔM15 for blue/white color screening of recombinant clones
endA1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by Endonuclease I
recA1 for reduced occurrence of non-specific recombination in cloned DNA
One Shot® TOP10 Kits are available with either chemically competent or electrocompetent E. coli to fit your specific transformation needs. TOP10 E. coli are also available in the high-throughput MultiShot™ format.
Write a review
Sharing your experience will help scientists like you. Achieve Reviewer Status and Win an iPad air (All reviews published will be entered into the next drawing on May 30th 2014).
Why make an enquiry with SelectScience?
EASY It’s quick and simple to do
FAST Your enquiry will be delivered straight to the manufacturer
FREE You’re under no obligation
SECURE We only pass your details on to trusted suppliers at your request
SAVE TIME Submit your details once and make multiple enquiries