Preparative separations place high demands on silica based HPLC materials.

Apart from excellent selectivity and base deactivation, robustness (pH-, pressure-stability, …) and capacity are vital criteria for optimal and efficient separation at the preparative scale.

MACHEREY-NAGEL offers a silica based HPLC phase which matches all these needs. NUCLEODUR® C₁₈ HTec is the perfect choice for all analytical separations combined with ideal up-scaling options.

Selectivity and Base Deactivation
The innovative and special endcapping procedure leads to exceptionally good base deactivation – the Engelhardt test demonstrates superb selectivity, peak symmetry and peak shape over the entire polarity range. In addition NUCLEODUR® C₁₈ HTec scores in low bleed characteristics and is therefore highly suitable for LC/MS.

Stability & Lifetime
Based on fully synthetic and extremely robust NUCLEODUR® silica (totally spherical type B silica of 3rd generation), NUCLEODUR® C₁₈ HTec offers outstanding mechanical rigidity and is thus the perfect choice also for self-packing of prep-columns. The special surface modification and endcapping procedure result in high chemical stability even at extreme chromatographic conditions like high flow rates, temperature or critical solvents (DMSO). Furthermore, NUCLEODUR® C₁₈ HTec columns show a remarkably long lifetime in acidic (pH 1) as well as basic (pH 10) mobile phases.

Up-Scaling
Due to highest quality standards in our silica production and phase chemistry combined with optimised packing technology, NUCLEODUR® C₁₈ HTec delivers exceptional transferability from analytical to preparative scale. This doesn’t just apply to the use of different particle sizes (e.g. 5, 7 or 10 µm) but also for diverse column dimensions (e.g. ID 4.6 to 21 mm).

Capacity
A vital criterion for efficiency in preparative HPLC is the capacity of the separation medium. NUCLEODUR® C₁₈ HTec is characterised by a notably high loadability under both basic and acidic conditions.

Conclusion
Due to innovative surface coating procedures NUCLEODUR® C₁₈ HTec offers excellent analytical separation properties and is the first choice for up-scaling to preparative column dimensions.