Ni Sepharose 6 Fast Flow

Ni Sepharose 6 Fast Flow
 5.0/5.0 (1 review)

GE Healthcare

Chromatography medium for purification of histidine-tagged proteins. Ni Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the ...read more

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Reviews

Anwar Ullah


Status:


See all reviews
Member since 2012

Job Title:
Post Doc / Research Fellow
Discipline:
Biochemistry
Work Field:
Academia / University
Organization:
UNESP/IBILCE
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RATING: 5.0

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 21 May 2014

Application Area: Protein Purification
Ni Sepharose 6 Fast Flow

"Ni Sepharose 6 Fast Flow produced by GE Healthcare Life Sciences is used for purification of His-tag proteins. The resin has high binding capacity (Approx. 40 mg histidine-tagged protein). The bind proteins can be eluted using 0.5 M imadazol solution. I recommend this resin to for purification of histidine-tagged protein."

Description

 

Chromatography medium for purification of histidine-tagged proteins.

Ni Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix and pre-charged with Ni2₊ ions for purification of histidine tagged proteins.

The coupling chemistry and the novel ligand is designed to give minimal ligand and Ni2₊ leakage while providing high capacity.

Ni Sepharose 6 Fast Flow
•Designed for scaling up purification of histidine-tagged proteins
•High protein binding capacity and minimal leakage of Ni2₊ ions
•As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
•Available in convenient prepacked HisPrep and HisTrap column formats.

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