Ni Sepharose 6 Fast Flow

Manufacturer GE Healthcare
  |  1 reviews

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Average Rating: 5.0
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  • Application Area: Protein Purification

"Ni Sepharose 6 Fast Flow produced by GE Healthcare Life Sciences is used for purification of His-tag proteins. The resin has high binding capacity (Approx. 40 mg histidine-tagged protein). The bind proteins can be eluted using 0.5 M imadazol solution. I recommend this resin to for purification of histidine-tagged protein."

Review date: 12 Oct 2015 | Ni Sepharose 6 Fast Flow
Chromatography medium for purification of histidine-tagged proteins.

Ni Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix and pre-charged with Ni2₊ ions for purification of histidine tagged proteins.

The coupling chemistry and the novel ligand is designed to give minimal ligand and Ni2₊ leakage while providing high capacity.

Ni Sepharose 6 Fast Flow
•Designed for scaling up purification of histidine-tagged proteins
•High protein binding capacity and minimal leakage of Ni2₊ ions
•As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
•Available in convenient prepacked HisPrep and HisTrap column formats.