Ni Sepharose 6 Fast Flow

Chromatography medium for purification of histidine-tagged proteins.

Ni Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix and pre-charged with Ni2₊ ions for purification of histidine tagged proteins.

The coupling chemistry and the novel ligand is designed to give minimal ligand and Ni2₊ leakage while providing high capacity.

Ni Sepharose 6 Fast Flow
•Designed for scaling up purification of histidine-tagged proteins
•High protein binding capacity and minimal leakage of Ni2₊ ions
•As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
•Available in convenient prepacked HisPrep and HisTrap column formats.

Receive your quote directly from GE Healthcare for Ni Sepharose 6 Fast Flow

Reviews

1 Scientist has reviewed this product


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Average Rating: 5.0

Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
Rating: 5.0
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date: 21 May 2014

  • Application Area: Protein Purification

  • Ni Sepharose 6 Fast Flow

"Ni Sepharose 6 Fast Flow produced by GE Healthcare Life Sciences is used for purification of His-tag proteins. The resin has high binding capacity (Approx. 40 mg histidine-tagged protein). The bind proteins can be eluted using 0.5 M imadazol solution. I recommend this resin to for purification of histidine-tagged protein."

Description

Chromatography medium for purification of histidine-tagged proteins.

Ni Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix and pre-charged with Ni2₊ ions for purification of histidine tagged proteins.

The coupling chemistry and the novel ligand is designed to give minimal ligand and Ni2₊ leakage while providing high capacity.

Ni Sepharose 6 Fast Flow
•Designed for scaling up purification of histidine-tagged proteins
•High protein binding capacity and minimal leakage of Ni2₊ ions
•As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
•Available in convenient prepacked HisPrep and HisTrap column formats.

Product Overview

Product Image

Ni Sepharose 6 Fast Flow

by GE Healthcare

5.0/5.0 (1 reviews)
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5






Receive your quote directly from GE Healthcare for Ni Sepharose 6 Fast Flow