Purification of His•Tag fusion proteins by metal chelation chromatography
The His•Bind® family of products offers a wide selection of supports designed for rapid one-step purification of proteins containing the His•Tag® sequence by immobilized metal affinity chromatography (IMAC).
The His•Tag sequence (6, 8 or 10 consecutive histidine residues) binds to divalent cations (Ni2+) immobilized on NTA*- and IDA-based His•Bind resins. After unbound proteins are washed away, the target protein is recovered by elution with either imidazole or slight reduction in pH. This versatile system enables proteins to be purified under gentle, non-denaturing conditions, or in the presence of either 6 M guanidine or urea. Further details can be found in the His•Bind Purification Kits Overview. *manufactured by QIAGEN
Feedback
Request info
Write a review
Sharing your experience will help scientists like you. Achieve Reviewer Status and Win an iPad 3 (All reviews published will be entered into the next drawing on May 31st 2013).
