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Member since: 2015
Organization: The university of Iowa
Application Area: preparing proteins for trypsin digest & MS
"Pre-cast polyacrylamide gels NuPAGE Novex 4-12% Bis-Tris Gels usually have optimal separation of your small- to medium-sized proteins under denaturing conditions. It has a neutral pH environment so it minimizes protein modifications. I found NuPAGE Novex Bis-Tris gels is good for preparing proteins for trypsin digest and mass spectrometry. We usually run 50-60min to separate protein and protein separation is very good. There are well markers on each gel, so it is easy to load samples. Customer service is good. One box of gels is expensive, it is better sale 2 or 5 gel package."
Member since: 2012
Organization: Stony Brook University
"NativePAGE has an easy to use buffer system. Also, the apparatus for running the gels is great, gaskets don't leak and so the anode and cathode buffers don't mix. Furthermore, this system is reliable and easy to replicate."