Naive CD4+ T Cell Isolation Kit II
The Naive CD4+ T Cell Isolation Kit II provides a fast and efficient way to isolate untouched naive CD4+ T cells from human peripheral blood. It is the new generation of the Naive CD4+ T Cell Isolation Kit, for even higher purities of the isolated naive T helper cell population.
For magnetic depletion of memory CD4+ T cells and non-CD4+ T cells, PBMCs are incubated with a cocktail of biotinylated CD45RO, CD8, CD14, CD15, CD16, CD19, CD25, CD34, CD36, CD56, CD123, anti-TCRγ/δ, anti-HLA-DR, and CD235a (glycophorin A) antibodies. These cells are subsequently magnetically labeled with Anti-Biotin MicroBeads for depletion.