The CaPO4 method of DNA transfection was initially developed as a technique for measuring the infectivity of isolated viral DNA. The precipitate formed by CaPO4 enables transfection by enhancing the adsorption of DNA to cell membranes, thus facilitating the DNA uptake by mammalian cells.
>100-fold increase in transient transfection efficiency
Increases yield of stable transformants
Reduces total time for transient assay to as little as 12 hours
Modified bovine serum increases number of cells transfected
Unprecedented efficiency with retroviral and adenoviral systems
Promoter analysis of numerous constructs
Genetic screening protocols for identifying agents that modulate gene expression