Thermo Scientific™ MAbPac™ SCX-10 Monoclonal Antibody Analysis Column
MAbPac™ SCX-10 Columns for the High-Resolution Separation and Analysis of Monoclonal Antibody VariantsThe Thermo Scientific™ MAbPac™ SCX-10 column line has been expanded to include 3 and 5 μm particle sizes for fast, high-resolution analysis as well as rapid separation (RS) columns for bio-UHPLC separations. These smaller-particle-size columns provide the same high resolution as the 10 μm columns, but with…
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MAbPac™ SCX-10 Columns for the High-Resolution Separation and Analysis of Monoclonal Antibody Variants
The Thermo Scientific™ MAbPac™ SCX-10 column line has been expanded to include 3 and 5 μm particle sizes for fast, high-resolution analysis as well as rapid separation (RS) columns for bio-UHPLC separations. These smaller-particle-size columns provide the same high resolution as the 10 μm columns, but with significantly faster analysis time. In addition, salt or pH gradients can be used to provide fast high-resolution, high-throughput analysis of monoclonal antibody (MAb) variant samples.
The MAbPac™ SCX-10 columns are strong cation-exchange columns designed specifically for the high-resolution, high-efficiency analysis of MAbs and associated variants. The unique nonporous pellicular resin provides exceptionally high resolving power, permitting the separation of MAb variants that differ by as little as one charged residue. Hydrophobic interactions with the resin are eliminated for very efficient peaks.
MAbPac™ SCX-10 Monoclonal Antibody Analysis Column Features:
- Unequalled high-resolution separations
- Exceptionally high efficiency
- Highest resolution of monoclonal antibody variants
- Unmatched column-to-column and lot-to-lot reproducibility
- Hydrophobic interactions eliminated
- Ideal for stability testing
Column Details:
- Chemistry: Sulfonic
- Particle Size: 3, 5, 10 μm
- Length: 50, 150, 250 mm
- ID: 2.0, 4.0, 4.6 mm
Development of Ultra-Fast pH-Gradient Ion Exchange Chromatography for the Separation of Monoclonal Antibody Charge Variants
The pre-formulated buffers for pH gradient, introduced by Thermo Fisher Scientific, have greatly simplified the development of ion exchange chromatography (IEX) of monoclonal antibodies (mAbs). This application note shows how to push the throughput of pH gradient IEX even further. To achieve this, a MAbPac SCX-10 RS column, 5 μm, 2.1 × 50 mm was operated on a new Thermo Scientific™ Vanquish™ UHPLC system. The Vanquish UHPLC is a fully biocompatible system, suitable for the analysis of intact proteins.
How Does the Analysis of Biomolecules Benefit from UHPLC?
Ultra high performance liquid chromatography (UHPLC) provides improved separation speed, throughput, and sensitivity by employing stationary phase particles of around 2 μm or smaller. Biomolecules have, apart from their size, other differences from small molecules (e.g. charges, complex structures) that make the application of UHPLC less straightforward. This article discusses the rationale of using UHPLC in life sciences. It also discusses the requirements for a true bio UHPLC system and shows examples of relevant biomolecule separations.
High-Throughput, High-Resolution Monoclonal Antibody Analysis with Small Particle Size HPLC Columns
This application note shows the selectivity differences between ProPac WCX-10 and MAbPac SCX-10 phases for protein separation. Applications using smaller particle columns for high-throughput, high-resolution mAb separations are also described. The ruggedness of small particle phase columns is also presented.
A Fast and Robust Linear pH Gradient Separation Platform for Monoclonal Antibody (mAb) Charge Variant Analysis
This application note describes a fast, 10 min cycle time separation of mAb charge variants using a linear pH gradient separation method.
Rugged HPLC Column Supports Biotherapeutic Drug Development
New HPLC column designed for exceptional resolution in antibody characterization and separation
