Lightning-Link™ Allophycocyanin (A-PC) / C-Phycocyanin (C-PC) / R-Phycocyanin (R-PC) kit
Say goodbye to secondary antibodies – Go direct using the World’s Easiest DIY Antibody Labeling Kit - Lightning-Link™!
• Eliminates need for secondary antibodies
• Reduces the number of wash steps
• Ideal for multi-protein analysis – with over 40 available labels
• Very easy to use
• 100% antibody recovery
The DIY Lightning-Link™ kit allows you to label an antibody with minimal hands-on time - less than 30 seconds. Simply add your antibody solution to a tube of Lightning-Link™ mix and the label becomes covalently attached to your antibody in a gentle controlled process. It is the easiest and quickest method available for antibody conjugation.
Direct antibodies labelling greatly simplifies immunodetection techniques. Without the problems of crossover and/or non-specific binding from secondary reagents it is far easier to obtain high quality, pioneering results in techniques such as flow cytometry, ELISA, Western blotting and immunohistochemistry. A reduction in the number of incubation and wash steps also saves you time and money.
Antibody conjugates in Lightning-Link™ Phycocyanin Antibody Labeling Kits:
Allophycocyanin (APC) is a large 105kDa fluorescent phycobiliprotein commonly used in FACS analysis. It absorbs at 650nm and emits at 662nm, but can be excited by either a dye laser (595-605nm), or a HeNe laser (633nm). APC has a high absorption coefficient of 7.0 x 105 cm-1 M-1 and quantum efficiency (0.68).
C-Phycocyanin is a lager 232kDa phycobiliprotein isolated from Spirulina sp, a blue green algae. Like other phycobiliproteins CPC is fluorescent, with a high absorption coefficient (2.5 x 106 cm-1 M-1), high quantum yield (0.84) and large stokes shift. Its absorption peak is at 620nm and its emission peak is at 647nm.
R-Phycocyanin (R-PC) is a 100kDa phycobiliprotein isolated from red algae. It has an absorption peak at 617nm and an emission peak at 633nm. The protein has an extinction coefficient of 1.5 x 105 cm-1 M-1