Pure recombinant high-fidelity DNA polymerase from Thermococcus kodakaraensis KOD1KOD HiFi DNA Polymerase* is a recombinant form of Thermococcus kodakaraensis KOD1 DNA polymerase. KOD HiFi is a high fidelity thermostable polymerase that amplifies target DNA up to 6 kbp in length with superior accuracy and yield. The enzyme’s 3′→5′ exonuclease-dependent proofreading activity results in a lower PCR mutation frequency than any other commercially available DNA polymerase. The elongation rate and processivity are 5 times and 10 to 15 times higher, respectively, than Pfu DNA polymerase, resulting in highly accurate and robust yield in a short reaction time. The enzyme generates blunt-ended PCR products suitable for cloning with Novagen’s Perfectly Blunt® and LIC Vector Ki...Read more
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