KOD HiFi DNA PolymeraseEMD Millipore, a division of Merck KGaA, Darmstadt, Germany
Pure recombinant high-fidelity DNA polymerase from Thermococcus kodakaraensis KOD1
KOD HiFi DNA Polymerase* is a recombinant form of Thermococcus kodakaraensis KOD1 DNA polymerase. KOD HiFi is a high fidelity thermostable polymerase that amplifies target DNA up to 6 kbp in length with superior accuracy and yield. The enzyme’s 3′→5′ exonuclease-dependent proofreading activity results in a lower PCR mutation frequency than any other commercially available DNA polymerase. The elongation rate and processivity are 5 times and 10 to 15 times higher, respectively, than Pfu DNA polymerase, resulting in highly accurate and robust yield in a short reaction time. The enzyme generates blunt-ended PCR products suitable for cloning with Novagen’s Perfectly Blunt® and LIC Vector Kits.
Unit definition: one unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 minutes at 75°C, in a reaction containing 20 mM Tris-HCl (pH 7.5 at 25°C), 8 mM MgCl2, 0.5 mM DTT, 50 mg/ml BSA, 150 µM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [3H]dTTP) and 150 µg/ml activated calf thymus DNA.
Higher fidelity than Pfu DNA polymerase—excellent for cloning
Greater yield—extension speed is 2X faster than Taq DNA polymerase and 5X faster than Pfu DNA polymerase
More accurate PCR in a shorter time
Higher processivity—sequential nucleotide polymerization is 10- to 15-fold greater than Pfu and Tli DNA polymerases
No truncated amplification products