Thermo Scientific Klenow Fragment is the large fragment of DNA polymerase I. It exhibits 5'→3' polymerase activity and 3'→5' exonuclease (proofreading) activity, but lacks 5'→3' exonuclease activity of DNA polymerase I.
Highlights • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, aminoallyl-, biotin-, digoxigenin- and fluorescently-labeled nucleotides) • Active in restriction enzyme, PCR, RT, and T4 DNA Ligase buffers
Applications • DNA blunting by fill-in 5'-overhangs • Random-primed DNA labeling • Labeling by fill-in 5'-overhangs of dsDNA • DNA sequencing by the Sanger method • Site-specific mutagenesis of DNA with synthetic oligonucleotides • Second strand synthesis of cDNA
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