Kinase Glo® Assay

Kinase Glo® Assay
by Promega Corp.
 4.6/5.0 (1 review)

Universal, HTS Kinase Screening Assays Use higher ATP concentrations: Linear response up to 500μM ATP. Use any kinase and kinase-substrate combination, including peptide, protein, lipid, and sugar substrates. Perform the assay without substrate modifications. Obtain reliable results: Z´-factor values routinely >0.7. Reduce false hits: Luminescence is much less suscept...read more

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AVERAGE RATING: 4.6

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  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY

Magdalena Przydzial


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Member since 2011

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Center, Computational Sci
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RATING: 4.6

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 21 Jun 2011

Kinase Glo® Assay

"we analyze data generated from this assay."

Description

 

Universal, HTS Kinase Screening Assays

  • Use higher ATP concentrations: Linear response up to 500μM ATP.
  • Use any kinase and kinase-substrate combination, including peptide, protein, lipid, and sugar substrates.
  • Perform the assay without substrate modifications.
  • Obtain reliable results: Z´-factor values routinely >0.7.
  • Reduce false hits: Luminescence is much less susceptible to interference from library compounds than fluorescence-based assays.

How It Works

The kinase reaction is conducted under the appropriate conditions. ATP remaining at the time that the Kinase-Glo® Reagent is added is used as a substrate by the Ultra-Glo™ Luciferase to catalyze the mono-oxygenation of luciferin and the generation of light. Luminescence is inversely related to kinase activity.

Linear out to 500μM ATP

The Kinase-Glo® Platform consists of three assay formats: the Kinase-Glo® Assay, which is used to monitor kinase activity using up to 10μM ATP; the Kinase-Glo® Plus Assay, which is used for assays requiring higher ATP concentrations (up to 100μM); and the Kinase-Glo® Max Assay, which is used for assays requiring up to 500μM ATP.

Perfect for HTS applications

Z´-factor is a statistical value that compares the assay dynamic range to data variation in order to assess assay quality. Z´-factors greater than 0.5 indicate excellent assay quality.

Uncover non-ATP binding site inhibitors

Use the Kinase-Glo Assays to distinguish ATP competitive inhibitors from noncompetitive inhibitors. Use PKA inhibitor (noncompetitive, PKI, and competitive, H89) titrations were performed using the Kinase-Glo Plus Assay. IC50 results determined using Kinase-Glo Plus varied just 2-fold for the noncompetitive inhibitor PKI (3.5nM vs. 7.9nM) at 10 and 100μM ATP, respectively. The IC50 results varied 7-fold for the competitive inhibitor H89 (0.06μM vs. 0.4μM) at 10 and 100μM ATP, respectively.

Product Overview

Kinase Glo® Assay by Promega Corp.
Kinase Glo® Assay




  • 5out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY





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