iScript™ Advanced cDNA Synthesis Kit for RT-qPCR (170-8842)

Manufacturer Bio-Rad
  |  1 reviews

Product Image

Average Rating: 3.0
1 Scientist has reviewed this product

4 out of 5
Ease of use
4 out of 5
After sales service
1 out of 5
Value for money

Rating: 3.0

  • Application Area: Neuroscience

"There is a lot of information about this synthesis product. The kit is useful when trying to obtain the best quality DNA."

Review date: 27 Nov 2012 | iScript™ Advanced cDNA Synthesis Kit for RT-qPCR (170-8842)
The iScript Advanced cDNA Synthesis Kit for RT-qPCR is an enhanced formulation that delivers increased data throughput from a single 20 µl reverse transcription (RT) reaction for real-time qPCR. This two-tube kit enables superior RT capacity of input RNA up to 7.5 µg.

Key Features and Benefits:

  • Increase qPCR data throughput and cost effectiveness from a single reaction — cDNA synthesized from higher input RNA amounts can be used for analyzing large numbers of target genes
  • Detect low-level target genes — uncompromised sensitivity even with less input RNA for when RNA sample is limited
  • Reduce inter-assay variability and improve reproducibility — eliminate the need for multiple cDNA synthesis reactions
  • Allow true representation of target mRNA levels — potent blend of RNase inhibitor prevents RNA degradation during reaction setup and RT
  • Increase primer design flexibility and prevent 5' and 3' bias — optimum blend of oligo(dT) and random primers for superior RNA coverage
  • Set up reactions with ease and obtain cDNA in less time — 2-tube kit generates cDNA in 35 min