The innuPREP Plasmid Mini Kit allows researchers to isolate plasmid DNA from bacterial lysates quickly and easily.
The method combines alkaline lysis with the process of binding plasmid DNA to a filter membrane once the chromosomal DNA and bacterial proteins have been precipitated. The bound plasmid DNA are then washed and eluted by adding a low salt buffer. The resulting isolated plasmid DNA can then be used immediately in a variety of additional downstream applications. The new method allows scientists to isolate plasmid DNA from 0.5–10 ml starting material. The use of a novel binding membrane makes it possible to isolate up to 40 µg of pDNA.
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