innuPREP Plasmid Mini Kit

innuPREP Plasmid Mini Kit
 4.3/5.0 (1 review)

Analytik Jena Life Science

The innuPREP Plasmid Mini Kit allows researchers to isolate plasmid DNA from bacterial lysates quickly and easily. The method combines alkaline lysis with the process of binding plasmid DNA to a fil...read more

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Reviews

Angela Jablonski


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Member since 2013

Organization:
University Of Pennsylvania
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RATING: 4.3

  • 5 out of 5EASE OF USE
  • 3 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 02 Dec 2013

Application Area: Purify Plasmid DNA
innuPREP Plasmid Mini Kit

"This is a good alternative to the Qiagen kit but quicker and much more cost effective. I received the same level of yield and purity as I have with other competitors' products, and DNA worked well with an extra-chromosomal array."

Description

 

The innuPREP Plasmid Mini Kit allows researchers to isolate plasmid DNA from bacterial lysates quickly and easily. The method combines alkaline lysis with the process of binding plasmid DNA to a filter membrane once the chromosomal DNA and bacterial proteins have been precipitated. The bound plasmid DNA are then washed and eluted by adding a low salt buffer. The resulting isolated plasmid DNA can then be used immediately in a variety of additional downstream applications. The new method allows scientists to isolate plasmid DNA from 0.5 – 10 ml starting material. The use of a novel binding membrane makes it possible to isolate up to 40 µg of pDNA.

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