innuCONVERT Bisulfite Kits by Analytik Jena GmbH+Co. KG

• Complete conversion of non-methylated cytosine to uracil in just a few hours
• Flexible for use in all commonly used thermocylers or heating blocks
• Denaturation and conversion combined in a single work sequence
• Automized desulfonation and purification for usage of InnuPure® C16 and up to 16 samples in parallel
• Total time: 85 minutes

The innuCONVERT Bisulfite Purification Kit-IPC16 allows users to completely convert nonmethylated cytosine to uracil in just a few hours. DNA sample denaturation and bisulfite treatment are combined in the same reaction vessel. After a total reaction time of approx. 45 minutes, the converted DNA is isolated and desulfonated using the InnuPure® C16. It is possible to purify 16 samples in parallel. The eluted DNA is then ready-to-use in downstream applications (such as PCR, sequencing, etc.).

innuCONVERT Bisulfite Basic Kit

• Complete conversion of non-methylated cytosine to uracil in just a few hours
• Flexible for use in all commonly used thermal cyclers or heating blocks
• Denaturation and conversion combined in a single work sequence

The innuCONVERT Bisulfite Basic Kit allows users to completely convert non-methylated cytosine to uracil in just a few hours. DNA sample denaturation and bisulfite treatment are combined in the same reaction vessel. After a total reaction time of approx. 3 hours, the converted DNA is isolated and desulfonated in a column. The eluted DNA is then ready-to-use in downstream applications (such as PCR, sequencing, etc.).

innuCONVERT Bisulfite All-In-One Kit

• DNA extraction for different starting materials
• Includes a module for completely converting non-methylated cytocine to uracile in a few minutes
• Liquid reagents are stable and can be stored
• Denaturation and conversion combined in a single work sequence
• Overall optimized process is complete in under 5 hours

The innuCONVERT Bisulfite All-In-One Kit al-lows researchers to extract DNA from a variety of relevant starting materials and then completely convert non-methylated cytosine to uracil in a few hours. A specially optimized universal buffer can be used for processing different lysis protocols, while DNA sample/lysate denaturation and bisulfite treatment take place in a single reaction vessel. This is followed by on-column purification and desulfonation of the converted DNA. The pure nucleic acid obtained can then be easily used for later downstream applications (such as PCR, sequencing).

innuCONVERT Bisulfite Body Fluids Kit

• Enrichment and extraction of free-circulating DNA from body fluids (3 mL)
• Easy to handle, highly efficient and fast (< 3.5 h)
• Includes a module for completely converting non-methylated cytocine to uracil
• Liquid reagents for stable storage
• Optimized workflows: denaturation and conversion in a single step

The innuCONVERT Bisulfite Body Fluids Kit is a unique newcomer to the market for fast, accurate bisulfite conversion. This is the first kit that both isolates cell-free DNA from an exceptionally wide variety of body fluids and then completely converts non-methylated cytosine to uracil. A capacity for up to 3 mL serum, plasma, cell culture supernatants, ascites, pleural effusions, and other similar substances in conjunction with the ideal enrichment method (PME – Polymer Mediated Enrichment) yields top-of-the-line sensitivity. The entire process, including cell-free DNA extraction, a coupled denaturation/bisulfite conversion step, desulfonation and subsequent purification of the converted DNA (using a Spin Filter column), is complete in just a few hours. Direct downstream applications are available immediately.