The Guava RapidQuant™ Mouse IgG Kit provides quantitation of Mouse IgG antibodies, typically found in hybridoma supernatants, using a bead-based assay followed by analysis on the Guava EasyCyte™ or Ea...read more
The Guava RapidQuant™ Mouse IgG Kit provides quantitation of Mouse IgG antibodies, typically found in hybridoma supernatants, using a bead-based assay followed by analysis on the Guava EasyCyte™ or EasyCyte™ Plus System. The kit contains IgG Capture Beads (Protein Gbound beads), which bind mouse antibodies (analyte) through the Fc region. The bound analyte is detected and quantitated using the FITC-labeled goat anti-mouse IgG antibody provided in the kit. A mouse antibody standard (whole IgG), also provided with the kit, is used at different concentrations to obtain a standard curve. The kit quantitates IgG1, IgG2a, and IgG2b antibodies in the range of 2.5-40 μg/ mL using the single standard provided. It also detects IgG3, but at a lower efficiency than the othersubtypes. This kit works only with intact antibodies that include an Fc region. If the antibody to be tested is an F(ab')2, Fab, sFv, or other variant without an Fc region, this kit should not be used.
Write a review
Sharing your experience will help scientists like you. Achieve Reviewer Status and Win an iPad air (All reviews published will be entered into the next drawing on May 30th 2014).
Why make an enquiry with SelectScience?
EASY It’s quick and simple to do
FAST Your enquiry will be delivered straight to the manufacturer
FREE You’re under no obligation
SECURE We only pass your details on to trusted suppliers at your request
SAVE TIME Submit your details once and make multiple enquiries