The Guava RapidQuant™ Mouse IgG Kit provides quantitation of Mouse IgG antibodies, typically found in hybridoma supernatants, using a bead-based assay followed by analysis on the Guava EasyCyte™ or EasyCyte™ Plus System. The kit contains IgG Capture Beads (Protein Gbound beads), which bind mouse antibodies (analyte) through the Fc region. The bound analyte is detected and quantitated using the FITC-labeled goat anti-mouse IgG antibody provided in the kit. A mouse antibody standard (whole IgG), also provided with the kit, is used at different concentrations to obtain a standard curve. The kit quantitates IgG1, IgG2a, and IgG2b antibodies in the range of 2.5-40 μg/ mL using the single standard provided. It also detects IgG3, but at a lower efficiency than the othersubtypes. This kit works o...Read more
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