GoTaq® 2-Step RT-qPCR System by Promega Corp.

Manufacturer Promega Corp.
  |  2 reviews

GoTaq® 2-Step RT-qPCR System by Promega Corp. product image
GoTaq® 2-Step RT-qPCR System
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Average Rating: 4.4
2 Scientists have reviewed this product

5 out of 5
Ease of use
4 out of 5
After sales service
5 out of 5
Value for money

Rating: 5.0

  • Application Area: Cancer Research

"GoTaq® 2-Step RT-qPCR System is a kind of product I recommend to any PCR routine laboratory; It made my work easy and I had good results using it. "

Review date: 06 Oct 2015 | GoTaq® 2-Step RT-qPCR System
  • Status:

    Advanced Reviewer

  • Member since: 2007

  • Organization: King Abdullah International Medical Research Center

  • Ease of use
    4 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
Rating: 3.7

  • Application Area:Real Time PCR

"This kit is super easy to use and setup from RNA to qRT-PCR. My only concern was the need of standard RNA which I didn’t have. If they can include it in the kit, it will be fantastic."

Review date: 15 May 2014 | GoTaq® 2-Step RT-qPCR System
GoTaq®2-Step RT-qPCR System is a reagent system for quantitative analysis of RNA using a two-step reverse transcription-quantitative PCR (RT-qPCR) method.

The first step consists of cDNA synthesis using the GoScript™ Reverse Transcription System. Optimized reagents and ultra-active, GoScript™ Reverse Transcriptase result in high-efficiency, full-length cDNA synthesis from rare and abundant targets with up to 5 µg of RNA input. In the second step, cDNA is quantified using the GoTaq®qPCR Master Mix.

Formulated with a hot-start DNA polymerase, the GoTaq® qPCR Master Mix is optimized for reproducibly specific, sensitive, highly efficient qPCR using the standard or fast mode of a real-time PCR instrument. The GoTaq® qPCR Master Mix contains BRYT™ GREEN dye which can be twice as bright as of SYBR® Green I. The superior performance of these two steps allows researchers to observe earlier quantification cycle values over broader linear ranges.

The GoTaq® 2-Step RT-qPCR System provides a ready-to-use kit for analyzing a wide range of RNA targets by combining the high-activity of GoScript™ Reverse Transcription System with the ultra-bright fluorescence of GoTaq® qPCR Master Mix. The system is robust, requires little optimization, is flexible for a variety of applications, and carries the PCR performance guarantee.