Error-prone PCR is a random mutagenesis technique for introducing amino acid changes into proteins. Mutations are deliberately introduced during PCR through the use of error-prone DNA polymerases and reaction conditions.
EZ Clone version provides a fast 3-step cloning method and eliminates the need to have specific restriction sites
More uniform mutational spectrum in which mutations at A's and T's occur at the same frequency as G's and C's
Superior yields over a wide target range - 0.1 to 3.5 kb
Simple protocol offers easy control of mutation frequency
Efficient mutagenesis rates of 1 to 16 bases per kb per PCR