DyNAmo™ SYBR® Green 2-step qRT-PCR Kit enables reproducible detection and quantification of RNA with great sensitivity and specificity. The kit contain all necessary reagents for cDNA synthesis from various RNA sources and for subsequent qPCR. Both random primers and oligo(dT) primers are included in the kit. Alternatively, the user can use gene specific primers. The reverse transcriptase in the kit is M-MuLV RNase H+, which provides higher sensitivity to qPCR than RNase H- reverse transcriptases. The performance of the qPCR step is based on a hot start version of a modified Thermus brockianus DNA polymerase. A non-specific DNA-binding domain has been fused to the polymerase, which lends physical stability to the polymerase-DNA complex.
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