This DELFIA® GTP-binding kit is intended for the measurement of G-protein coupled receptor activation in membrane preparations. The DELFIA GTP-binding assay is based on GDP-GTP exchange on G-protein subunits followed by GPCR activation by agonists. There are thorough instructions and tools provided in the kit to optimize the assay for the membrane receptor of your interest.
The DELFIA GTP-binding kit's novel GTP-Eu-label is fluorescent and non-hydrolyzable, enabling the simple and robust monitor of the agonist-dependent activation of G-proteins without the addition of Enhanchement Solution. The europium-labeled GTP is also available separately as a bulk reagent (for 100x96 wells), supplied lyophilized.
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