Transferring exogenous DNA into E. coli is a standard laboratory method for cloning genes and constructing cDNA, genomic and epitope libraries. The limiting factor in many library-screening efforts or multiple fragment ligations is the efficiency by which DNA can be introduced into E. coli. Electroporation is one method to efficiently introduce DNA into E. coli.
CloneCatcher™ Gold are based on a proprietary method that allows rapid development and testing of new strains that are task specific. The resulting mutants have optimized performance profiles that produced substantially enhanced results.
CloneCatcher Gold Features:
CloneCatcher™ Gold with a guaranteed minimum efficiency between 8 x 1010 and 1.2 x 1011*
Construct libraries of greater complexity than ever before
Library construction possible with small amounts of input DNA
Use less topoisomerase-loaded vector and reduce overall cost