The CD4+CD62L+ T Cell Isolation Kit II has been developed for the improved isolation of CD4+CD62L+ T helper cells from spleen and lymph nodes. The new kit allows the isolation of naive CD4+ T cells with even better purity and recovery due to refinement of the depletion cocktail, including the addition of a CD25 and an anti-TCRγ/δ+ antibody. CD62L (L-selectin) is highly expressed on naive T cells and down-regulated upon activation. It is also expressed on a small subset of memory T helper cells, the central memory T cells, which can be distinguished from naive T helper cells by their high expression of CD44. Furthermore, CD62L is expressed on most thymocytes, naive CD8+ T cells, B cells, dendritic cells, macrophages, NK cells, neutrophils, eosinophils, regulatory T cells, and TCRγ/δ+ T cel...Read more
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