The CD4+ T Cell Isolation Kit II is an indirect magnetic labeling system for the isolation of untouched CD4+ T helper cells from human PBMCs. Non-CD4+ T cells, i.e., CD8+ T cells, γ/δ T cells, B cells, NK cells, dendritic cells, monocytes, granulocytes, and erythroid cells, are indirectly magnetically labeled by using a cocktail of biotin-conjugated antibodies against CD8, CD14, CD16, CD19, CD36, CD56, CD123, TCR γ/δ, and CD235a (glycophorin A). These cells are subsequently magnetically labeled with Anti-Biotin MicroBeads for depletion. Thereby, highly pure CD4+ T cells are obtained.
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