Components of the Upstate (Millipore) Casein Kinase 2 Assay Kit:
• Casein Kinase 2 Substrate Peptide (Cat.# 12-330)
• Assay Dilution Buffer I (ADBI) (Cat.# 20-108)
• PKA Inhibitor Cocktail (Cat.# 20-114)
• Magnesium/ATP Cocktail (Cat.# 20-113)
• P81 Phosphocellulose Squares (Cat.# 20-134)
For use to measure the phosphotransferase activity of Casein Kinase 2 (CK-2) in immunoprecipitates and column fractions based on phosphorylation of a specific substrate (CK-2 substrate peptide) using the transfer of the gamma-phosphate of [g-32P] ATP by CK-2 kinase