The high cellularity of cardiac tissue presents problems in permeabilization, so the CardioTACS Kit comes with two permeabilization reagents to provide options. The kit is based on DNA end-labeling using terminal deoxynucleotidyl transferase (TdT) and a modified nucleotide that is subsequently detected using our TACS Blue Label™ detection system.
Trevigen has developed an exclusive apoptosis grade TdT enzyme that coupled with the TACS Blue Label solution provides labeling 20 to 50 times more sensitive than standard diaminobenzidine (DAB) labeling methods. To ensure ease of data interpretation when the numbers of apoptotic cells are low, CardioTACS provides Nuclear Fast Red Counterstain which provides superb contrast to the TACS Blue Label in apoptotic cells. In addition, the kit includes TACS-Nuclease™ that is used to generate a positive control in your own sample. The positive control provides an internal control for permeabilization and labeling so optimization is nominal and the data can be interpreted with confidence.
These complete kits provides all the reagents required for labeling including two permeabilization reagents, labeling and stop buffers, labeling and detection reagents, TACS-Nuclease reagent, and a protocol detailing incubation times and reagent concentrations.
Components: Permeabilization reagents Labeling enzyme Chromogenic substrate Labeling buffer Optimized cation Counterstain Nucleotide mix Detection reagents TACS-Nuclease Items Not Included: Reagents for dehydration and rehydration, H2O2, PBS buffer, and disposables. Related Products : Cell Proliferation AssaysDNA Laddering kitsServices and Assessories Storage: Components are stored at -20°C, 4°C, and room temperature.