* Slices maintained for many hours prior to transfer to recording chamber
* Simple to set up and maintain
* Many slices supported on FOUR quick-change nylon nets

The Brain Slice Keeper 4 has been designed to pre-incubate brain slices prior to transfer into recording chambers. It consists of FOUR pairs of closely fitting acrylic rings, upper and lower, between which is wedged a sheet of nylon netting. In operation, it is totally immersed in the ACSF-filled holding vessel to which a 95% O2, 5% CO2 gas mixture is being supplied. The bubbles rising from the base of the holder saturate the ACSF and provide constant circulation of medium to the slices which rest on the nylon nets. Since the lower ring carries a deflector shield, bubbles are prevented from being trapped under the slices and ensure continuous circulation of medium. Slices remain viable for many hours in these conditions.