ASH is APA-based, full-length-enriched, single-stranded cDNA subtraction hybridization library.
- ASH cDNA subtraction is full-length-enriched and independent of PCR suppression.
- ASH cloning is directional and does not involve normalization.
- ASH method provides non-exponential amplification before subtraction because double-stranded cDNA is not required.
- ASH method has a better subtraction effect because 1) a high ratio of driver:tester can be used 2) normalization is not involved and 3) representation is well preserved before subtraction hybridization.