AequoScreen and AequoZen aequorin Cell Lines


PerkinElmer, Inc.

The AequoScreen™ technology is a generic GPCR technology which can be used with Gs, Gi and Gq coupled GPCRs and calcium coupled ion channels. The product portfolio includes: - Parental cell lines...read more

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Description

 

The AequoScreen™ technology is a generic GPCR technology which can be used with Gs, Gi and Gq coupled GPCRs and calcium coupled ion channels. The product portfolio includes:


- Parental cell lines (expressing mitochondrially targeted apoaequorin with / without the promiscuous G-protein Gα16)
- Double transfected cell lines (expressing mitochodrially targeted apoaequorin and GPCR with / without the promiscuous G-protein Gα16)

Following GPCR stimulation, increases in intracellular calcium enable measurement of the resulting flash luminescence signal.

Features and benefits include:

- Fully automatable and miniaturizable to 1536
- Enables reduction in reagents and consumable costs
- High signal to background ratio enabling detection of agonists, antagonists and allosteric modulators
- Robust assay (Z, CV values, solvent resistance)
- Reduces the number of false positives from autofluorescent compounds in screening libraries
- Option to work with cells in suspension to increase throughput
- Amenable for use with calcium coupled ion channels and tyrosine kinase receptors

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