AccuPrime™ Taq DNA Polymerase High Fidelity amplifies nucleic acid templates using antibody-mediated hot-start, a blend of Taq DNA Polymerase and proofreading enzyme, and AccuPrime™ accessory proteins for improved PCR fidelity, yield, and specificity over other hot-start DNA polymerases.With AccuPrime™ Taq DNA Polymerase High Fidelity you'll get:
- The highest specificity and yield for the most robust PCR amplification
- 9-fold higher fidelity than Taq DNA polymerase alone
- Mimimal optimization steps, even with non-optimized primer sets
- Efficient amplification of targets over a broad size range up to 20 kb
High fidelity is achieved by a combination of Platinum® anti-Taq DNA polymerase antibodies that inhibit polymerase activity, providing an automatic "hot-start", and the proofreading (3´a5´ exonuclease activity) enzyme Pyrococcus species GB-D. The thermostable AccuPrime™ View Moreaccessory proteins enhance specific primer-template hybridization during every cycle of PCR, preventing mispriming and enhancing PCR specificity and yield.