V3 Western Workflow™

Manufacturer Bio-Rad | View All Models  |  Available Worldwide
4.1
/
5.0
  |  9 reviews
The V3 Western Workflow combines traditional blotting techniques with innovative tools to rapidly v


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Average Rating: 4.1
9 Scientists have reviewed this product

4 out of 5
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money


Rating: 4.0

  • Application Area: Autoantibody discovery

"Quite effective product, makes the analysis easier and accurate. I can rely on this."

Review date: 10 Nov 2015 | V3 Western Workflow™ Complete System for Mini Gels (170-8292)
  • Status:

    Advanced Reviewer

  • Member since: 2014

  • Organization: Zydus Cadila VTC



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0

  • Application Area:Protein Transfer For Analysis

"Bio-Rad Western transfer system is very easy to use, as per my experience it takes only 45 mins- 60 minutes for blot transfer. More over as the buffer tank is made as single unit without joints as compared with other companies, there is no tension of buffer leak from it. The electrophoresis blotting module is very easy to handle as it has got lock and key function. In nut shell advantages of western blot unit. 1. Its buffer tank 2. Blotting module 3. Least buffer required 4. Less time consuming 5. Easy handling "

Review date: 06 Oct 2015 | V3 Western Workflow™
  • Status:

    Advanced Reviewer

  • Member since: 2015

  • Organization: BIOSS - University of Freiburg



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    3 out of 5
Rating: 3.7

  • Application Area:surface expression of B cell receptor

"This product is easy to use and saves a lot of your time by using auto settings of light exposure and best picture generation."

Review date: 28 Jul 2015 | V3 Western Workflow™ Complete System for Mini Gels (170-8292)
  • Status:

    Reviewer

  • Member since: 2013

  • Organization: St. John's University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0
"This is a high end product with excellent reproducibility! I would recommend it if you are using western blotting. You should definitely purchase this product as it will ensure experimental excellence."

Review date: 29 Nov 2013 | V3 Western Workflow™
  • Status:

    Reviewer

  • Member since: 2013

  • Organization: University of Illinois at Chicago



  • Ease of use
    3 out of 5
    After sales service
    4 out of 5
    Value for money
    3 out of 5
Rating: 3.3

  • Application Area:Biochemical Analysis For Kinase Activation

"It is a good, easy and quick way for western blot analysis. I wish it would be more sensitive for some low expression level protein detection. "

Review date: 29 Nov 2013 | V3 Western Workflow™


  • Status:

    Advanced Reviewer

  • Member since: 2010

  • Organization: Uni of Miss



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.3

  • Application Area:Signaling pathway studies

"This system is very useful for gel documentation and analysis of protein expression using western blotting technique. This system gives good qualitative images of expressed proteins and quantitative analysis data. It is also a very good system for DNA and RNA analysis. This system is easy to use and analyze the data using software for JPEG, TIF and original images to generate the qualitative and quantitative data for publications."

Review date: 29 Oct 2013 | V3 Western Workflow™
  • Status:

    Reviewer

  • Member since: 2013

  • Organization: Penn State Univ. College Of Medicine



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.0

  • Application Area:cancer biology

"The products are easy to use. The antibodies are of good quality and give consistent results every time they are used."

Review date: 03 Jun 2013 | V3 Western Workflow™
  • Status:

    Reviewer

  • Member since: 2013

  • Organization: NATIONAL CHENG KUNG UNIV.



  • Ease of use
    4 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
Rating: 3.7

  • Application Area:western blot

"I think it is a very useful tool to determine the interested protein expression. I used this equipment in my research. My research is about the regulation of cell cycle. I got clear and significant results from it."

Review date: 03 Jun 2013 | V3 Western Workflow™
  • Status:

    Reviewer

  • Member since: 2013

  • Organization: Ut Md Anderson Cancer Ctr.



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.3

  • Application Area:cancer research

"This product is easy to handle and sustainable. Results are clear and definite. Manuals are very easy to learn and follow even for new starters in the cancer biology. The system is not complicated therefore trouble shooting is always simple."

Review date: 03 Jun 2013 | V3 Western Workflow™

Bio-Rad's V3 Western Workflow is a five-step approach to streamlining your western blotting protocol. The V3 Western Workflow facilitates speed and validation at each step of a western blotting experiment — from running gels to quantifying proteins.

The complete V3 workflow incorporates traditional blotting techniques with innovative tools, such as TGX Stain-Free™ precast gels, the Trans-Blot® Turbo™ system, and the ChemiDoc™ MP imager, to quickly check electrophoresis results and blot transfer quality prior to western blotting, simplifying total protein quantitation analysis post-immunodetection to obtain results faster.

 

V3 Western Workflow™ 5 Step Workflow:

  1. Separate proteins — Mini-PROTEAN® TGX Stain-Free™ precast gels and Criterion™ TGX Stain-Free™ precast gels offer superior protein separation with fast run times and use standard Tris/glycine running buffers. TGX Stain-Free™ precast gels contain a unique technology formulated into the gel chemistry that enables rapid, high-quality separation of proteins in as little as 15 minutes.
  2. Visualize proteins — Protein separation can be visualized and confirmed using stain-free technology, which covalently binds proteins, after 1 minute of activation on a ChemiDoc MP imager. This allows the immediate visualization of proteins across the whole gel without staining. Stain-free technology is a sensitive, time-saving alternative to traditional Coomassie staining, offering a streamlined workflow.
  3. Transfer proteins — The Trans-Blot Turbo system is a rapid protein transfer apparatus that can reduce transfer protocols to as little as 3 minutes while maintaining high-efficiency protein transfers across a wide range of molecular weights.
  4. Verify protein transfer — The ChemiDoc MP imager paired with stain-free technology enables instant verification of protein transfer.
  5. Validate and quantitate — The ChemiDoc MP imager and Image Lab™ software may be used to validate western blotting data via total protein normalization in conjunction with housekeeping proteins. By normalizing to total protein, stripping and reprobing is no longer necessary. Image Lab software gives quantitative results instead of relative normalization.

Product Models Information


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V3 Western Workflow™ Complete System for Midi Gels (170-8293)

1708293

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V3 Western Workflow™ Complete System for Mini Gels (170-8292)

1708292  |

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V3 Western Workflow™ with Gel Doc™ EZ System for Midi Gels (170-8303)

1708303

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V3 Western Workflow™ with Gel Doc™ EZ System for Mini Gels (170-8302)

1708302

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Product Overview

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V3 Western Workflow™

Manufacturer Bio-Rad  |  Available Worldwide

3.9 / 5.0 | 9 reviews