V3 Western Workflow™

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V3 Western Workflow™
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 4.1/5.0 (6 reviews)

Bio-Rad

Bio-Rad's V3 Western Workflow is a five-step approach to streamlining your western blotting protocol. The V3 Western Workflow facilitates speed and validation at each step of a western blotting exper...read more

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Reviews

Charles Ashby


Status:


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Member since 2013

Organization:
St. John's University
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RATING: 5.0

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 29 Nov 2013

V3 Western Workflow™

"This is a high end product with excellent reproducibility! I would recommend it if you are using western blotting. You should definitely purchase this product as it will ensure experimental excellence."

Read 6 reviews

Ying He


Status:


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Member since 2013

Organization:
University of Illinois at Chicago
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RATING: 3.3

  • 3 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 3 out of 5VALUE FOR MONEY




Review date: 29 Nov 2013

Application Area: Biochemical Analysis For Kinase Activation
V3 Western Workflow™

"It is a good, easy and quick way for western blot analysis. I wish it would be more sensitive for some low expression level protein detection. "

Narayan Chaurasiya


Status:


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Member since 2010

Job Title:
Research Scientist / Senior / PI
Discipline:
Biochemistry
Work Field:
Academia / University
Organization:
University of mississippi
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RATING: 4.3

  • 5 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 29 Oct 2013

Application Area: Signaling pathway studies
V3 Western Workflow™

"This system is very useful for gel documentation and analysis of protein expression using western blotting technique. This system gives good qualitative images of expressed proteins and quantitative analysis data. It is also a very good system for DNA and RNA analysis. This system is easy to use and analyze the data using software for JPEG, TIF and original images to generate the qualitative and quantitative data for publications."

Arun K. Sharma


Status:


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Member since 2013

Organization:
Penn State Univ. College Of Medicine
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RATING: 4.0

  • 4 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 03 Jun 2013

Application Area: cancer biology
V3 Western Workflow™

"The products are easy to use. The antibodies are of good quality and give consistent results every time they are used."

YU-CHENG LEE


Status:


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Member since 2013

Organization:
NATIONAL CHENG KUNG UNIV.
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RATING: 3.7

  • 4 out of 5EASE OF USE
  • 3 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 03 Jun 2013

Application Area: western blot
V3 Western Workflow™

"I think it is a very useful tool to determine the interested protein expression. I used this equipment in my research. My research is about the regulation of cell cycle. I got clear and significant results from it."

Sun Jin Kim


Status:


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Member since 2013

Organization:
Ut Md Anderson Cancer Ctr.
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RATING: 4.3

  • 5 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 03 Jun 2013

Application Area: cancer research
V3 Western Workflow™

"This product is easy to handle and sustainable. Results are clear and definite. Manuals are very easy to learn and follow even for new starters in the cancer biology. The system is not complicated therefore trouble shooting is always simple."

Description

 

Bio-Rad's V3 Western Workflow is a five-step approach to streamlining your western blotting protocol. The V3 Western Workflow facilitates speed and validation at each step of a western blotting experiment — from running gels to quantifying proteins.

The complete V3 workflow incorporates traditional blotting techniques with innovative tools, such as TGX Stain-Free™ precast gels, the Trans-Blot® Turbo™ system, and the ChemiDoc™ MP imager, to quickly check electrophoresis results and blot transfer quality prior to western blotting, simplifying total protein quantitation analysis post-immunodetection to obtain results faster.


V3 Western Workflow™ 5 Step Workflow:

  1. Separate proteins — Mini-PROTEAN® TGX Stain-Free™ precast gels and Criterion™ TGX Stain-Free™ precast gels offer superior protein separation with fast run times and use standard Tris/glycine running buffers. TGX Stain-Free™ precast gels contain a unique technology formulated into the gel chemistry that enables rapid, high-quality separation of proteins in as little as 15 minutes.
  2. Visualize proteins — Protein separation can be visualized and confirmed using stain-free technology, which covalently binds proteins, after 1 minute of activation on a ChemiDoc MP imager. This allows the immediate visualization of proteins across the whole gel without staining. Stain-free technology is a sensitive, time-saving alternative to traditional Coomassie staining, offering a streamlined workflow.
  3. Transfer proteins — The Trans-Blot Turbo system is a rapid protein transfer apparatus that can reduce transfer protocols to as little as 3 minutes while maintaining high-efficiency protein transfers across a wide range of molecular weights.
  4. Verify protein transfer — The ChemiDoc MP imager paired with stain-free technology enables instant verification of protein transfer.
  5. Validate and quantitate — The ChemiDoc MP imager and Image Lab™ software may be used to validate western blotting data via total protein normalization in conjunction with housekeeping proteins. By normalizing to total protein, stripping and reprobing is no longer necessary. Image Lab software gives quantitative results instead of relative normalization.

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