Status:
Advanced ReviewerMember since: 2019
Organization: Universidad Nacional Autónoma de México
The best photodocumentation system on the market. An excellent investment.
Application Area: Analysis of the signaling pathways involved in apoptosis
"It is definitely the best on the market for acquisition and analysis of images of blots, both those obtained by chemiluminescence and those obtained by fluorescence. In addition, it is also the very versatile since you can photo-document protein gels stained with Coomasie, silver, etc., nucleic acid gels stained with ethidium bromide or with fluorochromes. In summary, the best."
Status:
ReviewerMember since: 2017
Organization: Cell Signaling Technolgoy, Inc.
Great product that has drastically increased productivity!
Application Area:Antibody testing and validation
"The Bio-Rad V3 workflow system has drastically increased productivity in my lab. The time savings from each step of the experiment has increased the throughput of our testing too. Going from essentially 5-6 hours for a Western to 2-3 hours has freed up time in the lab to focus on other areas of improvement too. The ChemiDoc imager produces high quality images too. I still believe that film is more sensitive, but the digital images are still more than sufficient for my needs. Also, its a much more sustainable solution to developing."
Status:
ReviewerMember since: 2014
Organization: Rowan University
Application Area:Autoantibody discovery
"Quite effective product, makes the analysis easier and accurate. I can rely on this."
Status:
Advanced ReviewerMember since: 2014
Organization: Zydus Cadila VTC
Application Area:Protein Transfer For Analysis
"Bio-Rad Western transfer system is very easy to use, as per my experience it takes only 45 mins- 60 minutes for blot transfer. More over as the buffer tank is made as single unit without joints as compared with other companies, there is no tension of buffer leak from it. The electrophoresis blotting module is very easy to handle as it has got lock and key function. In nut shell advantages of western blot unit. 1. Its buffer tank 2. Blotting module 3. Least buffer required 4. Less time consuming 5. Easy handling "
Status:
Advanced ReviewerMember since: 2015
Organization: BIOSS - University of Freiburg
Application Area:surface expression of B cell receptor
"This product is easy to use and saves a lot of your time by using auto settings of light exposure and best picture generation."
Status:
ReviewerMember since: 2013
Organization: St. John's University
"This is a high end product with excellent reproducibility! I would recommend it if you are using western blotting. You should definitely purchase this product as it will ensure experimental excellence."
Status:
ReviewerMember since: 2013
Organization: University of Illinois at Chicago
Application Area:Biochemical Analysis For Kinase Activation
"It is a good, easy and quick way for western blot analysis. I wish it would be more sensitive for some low expression level protein detection. "
Status:
Expert ReviewerMember since: 2010
Organization: Uni of Miss
Application Area:Signaling pathway studies
"This system is very useful for gel documentation and analysis of protein expression using western blotting technique. This system gives good qualitative images of expressed proteins and quantitative analysis data. It is also a very good system for DNA and RNA analysis. This system is easy to use and analyze the data using software for JPEG, TIF and original images to generate the qualitative and quantitative data for publications."
Status:
ReviewerMember since: 2013
Organization: Penn State Univ. College Of Medicine
Application Area:cancer biology
"The products are easy to use. The antibodies are of good quality and give consistent results every time they are used."
Status:
ReviewerMember since: 2013
Organization: NATIONAL CHENG KUNG UNIV.
Application Area:western blot
"I think it is a very useful tool to determine the interested protein expression. I used this equipment in my research. My research is about the regulation of cell cycle. I got clear and significant results from it."
Status:
ReviewerMember since: 2013
Organization: Ut Md Anderson Cancer Ctr.
Application Area:cancer research
"This product is easy to handle and sustainable. Results are clear and definite. Manuals are very easy to learn and follow even for new starters in the cancer biology. The system is not complicated therefore trouble shooting is always simple."
Bio-Rad's V3 Western Workflow is a five-step approach to streamlining your western blotting protocol. The V3 Western Workflow facilitates speed and validation at each step of a western blotting experiment — from running gels to quantifying proteins.
The complete V3 workflow incorporates traditional blotting techniques with innovative tools, such as TGX Stain-Free™ precast gels, the Trans-Blot® Turbo™ system, and the ChemiDoc™ MP imager, to quickly check electrophoresis results and blot transfer quality prior to western blotting, simplifying total protein quantitation analysis post-immunodetection to obtain results faster.
V3 Western Workflow™ 5 Step Workflow: