Sensitivity in a New Dimension: The New LSM 780 Laser Scanning Microscope
16 Oct 2009With the LSM 780, Carl Zeiss is now adding a new member to its family of LSM 7 laser scanning microscopes. The use of the new GaAsP detector technology has almost doubled the sensitivity of the currently leading LSM 710.
Cell biologists and neurobiologists can now visualize weakly fluorescent or bleach-sensitive specimens and specimens with fine structures more quickly and with higher image quality. The higher quantum efficiency of the new detectors makes it possible for the LSM 780 to also image specimens which could not be satisfactorily examined in the past, such as sensitive and weakly stained yeast cells.
To make the GaAsP detector technology available to a wide range of users, it will be offered in three versions: as an internal spectral 32-channel detector in the LSM 780 laser scanning microscope, as a detector upgrade for existing LSM 710 systems and as the external LSM BiG upgrade module. All versions of the 32-channel GaAsP array permit traditional spectral imaging, photon counting with maximum sensitivity, and single molecule visualization through fluorescence correlation spectroscopy (FCS) with one to six signal channels.
With the universal LSM BiG module, the GaAsP technology can be used for further imaging modes. For multiphoton microscopy with the LSM 780 NLO and 710 NLO systems, the LSM BiG significantly improves non-descanned detection. While in the past several specialized detectors were required for imaging, multiphoton examination and FCS, the GaAsP technology of the LSM BiG makes it possible for all applications to be carried out with one universal module—with better image quality and faster image capture.
Related Products
Request Quote for All Products
ZEISS LSM 780 Laser Scanning Microscope
ZEISS Research Microscopy SolutionsLSM 780 - Obtain Quantitative Information About Your Cells and Individual Molecules. The sensitivity of LSM 780 is quite simply outstanding. The GaAsP detector achieves 45 percent quantum efficiency compared to 25 percent typically by conventional PMT detectors. This results in accurate details and contrast-rich images of the challenging specimens you encounter in your live cell imaging.The system’s illumination and detection design allows you to simultaneously acquire up to ten dyes. You excite any common fluorophore with up to eight different lasers, detecting the signals with the 32 channel GaAsP detector. LSM 780 is so sensitive, the system even allows photon counting.The maximum possible acquisition speed available to you is determined by the sensitivity of your detection system. When you choose LSM 780, you benefit from new GaAsP detectors that bring a significant improvement on signal-to-noise ratio, allowing you to acquire your image sequences at least twice as fast.Parallel spectral detection also reads 34 channels simultaneously in Lambda mode: you acquire and separate up to ten colors simultaneously.LSM 780 allows you to achieve images of unparalleled detail, even from the most challenging specimens. The superior quality and sensitivity has been recognized by a distinguished R&D 100 Award in 2011.LSM 780 Laser Scanning Microscope Features: 32-channel GaAsP detector with a typical quantum efficiency of 45% – a significant improvement on the 25% typically achieved by classic PMT detectors. Potential for photon counting on GaAsP and the new side PMT. With lasers from 355 nm true UV to 660 nm far red, any dye will be excited optimally. Simple adaptation to changing acquisition strategies via the ZEN software’s Smart Setup. Improved ZEN software with modules for e.g. correlative microscopy, deconvolution and time lapse. Improved dynamics for better visualization of weak signals. Active cooling together with oversampling and photon counting modes deliver improved signal-to-noise ratio.