Product News: Life Technologies Launches qPCR and Capillary Electrophoresis General Purpose Reagents to Support the Advancement of Molecular Diagnostic Test Development

15 Nov 2013

Life Technologies Corporation announces the release of General Purpose Reagents (GPRs) for the development of molecular diagnostic assays based on qPCR and capillary electrophoresis (CE). GPR products are marked “For Laboratory Use” and comes with a higher quality assurance than Research Use Only products. They are also delivered with additional customer services such as tracking.

“As researchers begin translating genomic discoveries into actionable tests, they will need quality qPCR and sequencing components,” said Chris Linthwaite, President of Genetic Analysis at Life Technologies. Life Technologies is committed to providing the robust, high quality tools required for clinically oriented test development.”

“Access to high quality components listed with the FDA as General Purpose reagents is critical to our business as a diagnostics provider,” said Jeff D. Hester, Ph.D., Vice President of Research & Development at Viracor-IBT Laboratories. “These components help to ensure accurate and consistent results from our assays.”

GPRs for Capillary Electrophoresis

For capillary electrophoresis (for sequencing and fragment analysis), Life Technologies has introduced BigDye® Terminator Cycle Sequencing Reagents and BigDye® Direct Cycle Sequencing Reagents, as well as matrix and size standards, buffers and purification reagents.

BigDye® Terminator Cycle Sequencing Reagents provide the required components for cycle sequencing reactions in a premixed format. They are optimized for longer, higher-quality reads with uniform peak heights and optimal signal balance and are appropriate for a wide range of applications, including de novo sequencing and resequencing.

BigDye® Direct Cycle Sequencing Reagents provide highly flexible chemistry that is ideal for de novo sequencing, resequencing, and finishing with PCR products such as plasmids, fosmids, and BAC templates. These reagents offer a streamlined, time-saving process with all sequencing steps in a single reaction tube.

GPRs for qPCR Assay Development

For qPCR assay development, the TaqPath™ qPCR Master Mixes are compatible with both standard and fast cycling modes and are tolerant of inhibitors commonly found in clinical samples. In addition, the Master Mixes are functionally tested to ensure lot-to-lot reproducibility for CT consistency and wide dynamic range.

The TaqPath™ qPCR Master Mix, CG is specifically designed and optimized for gene expression and miRNA analysis. The TaqPath™ 1-Step RT-qPCR Master Mix, CG, is designed for rapid and reproducible pathogen detection and gene expression workflows. Now available as early access for evaluation, TaqPath GPRs are expected to be full commercialized prior to 2014.