EMD Millipore Introduces Magna ChIP® HiSens Chromatin Immunoprecipitation Kits
13 May 2013EMD Millipore, the life science division of Merck KGaA of Darmstadt, Germany, today introduced Magna ChIP® HiSens chromatin immunoprecipitation kits for superior enrichment and detection of specific regions of chromatin-associated DNA. These new kits enable target chromatin enrichment from both low and high amounts of input chromatin while delivering low backgrounds, high signal-to-noise ratios, and ultra-sensitive detection that is compatible with all commonly used downstream analysis applica¬tions including qPCR, next generation sequencing, and microarray.
Two versions of this new chromatin immunoprecipitation kit are available. The Magna ChIP® HiSens chromatin immunoprecipitation kit includes reagents, buffers, and beads for ChIP as well as materials for chromatin preparation and isolation from either cultured cells or tissues. The EZ-Magna ChIP™ HiSens chromatin immunoprecipitation kit includes positive and negative control antibodies and a validated qPCR primer set, in addition to the standard kit's components.
Unique to these kits is a single buffer system used for all sonication, ChiP, and wash steps. This streamlined approach, plus their specialized blend of protein A/G magnetic beads, not only allows ChIP from a wider range of antibody isotypes than kits with protein A or G beads alone, it also delivers lower backgrounds and higher fold enrichment. Unlike other approaches the Magna ChIP® HiSens kits are designed to allow ChIP analysis by qPCR without additional clean-up, reducing the time required to perform the protocol along with eliminating the potential for sample loss during clean-up.
Using these kits, researchers can ChIP from a wide range of input amounts (10,000 to 1,000,000 cells or cell equivalents) and get excellent performance starting from either cells or tissues. Performance of the Magna ChIP® HiSens kit was benchmarked against that of other low input ChIP kits. After strictly following the protocols for all kits evaluated, the Magna ChIP® HiSens kit showed significantly higher performance as measured by fold enrichment and background levels than competitor kits.
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Magna ChIP™ HiSens Chromatin Immunoprecipitation Kit
MerckThe Magna ChIP HiSens kit provides a complete set of validated, quality controlled reagents, and a detailed protocol to enable ChIP from a wide range of input amounts of chromatin obtained from either cells or tissues. Our specialized blend of protein A/G blend of magnetic beads is specifically produced for chromatin immunoprecipiptation and enables the use of a broader range of antibodies than protein A or G alone eliminating the need to purchase different kits for different antibody isotypes. The SCW Buffer is unique to the Magna ChIP HiSens kit and enables the use of a single buffer for multiple steps of the ChIP process (sonication, chromatin immunoprecipitation, and wash). The ChIP elution buffer provided with the HiSens kit has been formulated to allow analysis of enrichment by qPCR without additional clean-up steps for more rapid results. Features & Benefits: • Robust ChIP from as few as 10,000 to as many as 1,000,000 cells • Protocols and reagents for generation of chromatin from range of input sample type • Superior performance with variety of isotypes of either polyclonal or monoclonal antibodies • Specialized buffer and bead formulations; lower backgrounds and higher fold enrichment • Single buffer system for sonication, chromatin IP, and wash • Perform analysis of enrichment without additional purification after cross link reversal. • Compatible with all commonly used downstream analysis applications– qPCR, next generation sequencing, microarray