The assays are simple, can be used with any substrate type, and are ideal for both screening and enzyme characterization. The assays can also be applied to detecting other NDPs, including GDP and UDP and have been broadly applied to enzyme other than kinases, such as ATPases.
ADP Hunter Plus and ADP Hunter HS are designed for screening kinases and ATPases as they have low compound interference and may be used with up to 2mM DTT. These assays exhibit a broad ATP tolerance and are applicable to both high and low activity enzymes. ADP Hunter HS is a high sensitivity assay with very low background and a large signal to background ratio, enabling detection of as little as 300nM ADP.
• Endpoint (screening) and kinetic modes
• High sensitivity
• Any substrate type
Click on the article webpage to view the SBS07 poster.