Utrecht University Appointment to Advance Large Protein Research Using Orbitrap Technology

14 Nov 2013
Sarah Thomas
Associate Editor

The Executive Board of Utrecht University, by special appointment, named Alexander Makarov, Ph.D, professor of high resolution mass spectrometry at the Bijvoet Center of the Department of Chemistry.

Makarov is Director of Global Research, Life Science Mass Spectrometry (LSMS) for Thermo Fisher Scientific.

"I hope this appointment will become a bridge that connects academia to industry," Makarov said. "I want to make it easier for students and industrial scientists to learn from each other."

The new chair was created to advance research of new applications for analyzing large proteins and protein assemblies using Orbitrap technology. These may include detailed measurements of biologically important species, including biopharmaceuticals. Makarov lead the team that developed Orbitrap technology, which transformed a large sector of analytical science, and this appointment represents a strengthening of the existing research collaboration between the LSMS team and the proteomics group of Utrecht, which is lead by professor Albert Heck.

At the Bijvoet Center, pharmacists, chemists and biologists conduct research into and offer a research training program about the relationships between the structures and functions of biomolecules.

Thermo Scientific Exactive Plus Orbitrap LC-MS

Thermo Fisher Scientific

The Exactive Plus Orbitrap LC-MS is a benchtop full scan mass spectrometer designed for compound identification and high-throughput, high-performance screening. The Exactive Plus delivers high resolution accurate mass (HRAM) data and fast, reproducible results for qualitative and quantitative analysis. The availability of HRAM and full scan capability gives you all the data, all the time. It allows retrospective data analysis and eliminates MS method development leading to improved and simplified analytical throughput.Exactive Plus is easy-to-use, cost effective to operate and coupled with workflow-driven software, makes an ideal instrument for new users in routine analytical laboratories.Selected benefits of the Exactive Plus are specified below: High Resolving Power (up to 140,000) - Eliminates isobaric interferences to increase confidence for analysis or complex samples Mass accuracy - Better than 1ppm in full scan and AIF scan using fully automated AGC and mass calibration procedures offers confident identification U-HPLC Scan Speed - The 12Hz scanning frequency of Exactive Plus is fully compatible with U-HPLC and ensures exact mass measurement for fast chromatography applications Fast Polarity Switching - A full cycle of 1 positive and 1 negative scan is performed within 1 second at a resolution setting of 35,000 without sacrificing mass accuracy All-Ion-Fragmentation (AIF) - With multiple dissociation techniques; in source collisional induced dissociation (CID) and high energy collisional dissociation (HCD), provides MS and MS/MS data Full MS scan, AIF scan, data dependent AIF, alternating Full MS and AIF scan Intuitive software interface The Exactive Plus Orbitrap LC-MS is ideal for the following applications: Environmental and Food Safety Clinical Research Forensic Toxicology Drug discovery, metabolism and pharmacokinetics (DMPK) Metabolomics Pharmaceutical BioPharma – Intact Protein analysis Laboratories analyzing food, pharmaceuticals, neutraceuticals, and other complex samples can now purchase Exactive Plus LC-MS system equipped from the factory with IonSense ID-CUBE™ ion sources. This configuration is designed to minimize the need for sample preparation to simplify and speed analyses.

(20)

Tags